2BTJ

Fluorescent Protein EosFP - red form

This is a non-PDB format compatible entry.

Summary for 2BTJ

• Entry DOI: 10.2210/pdb2btj/pdb
• Descriptor: Green to red photoconvertible GFP-like protein EosFP (3 entities in total)
• Functional Keywords: photo-induced protein cleavage, green-to-red conversion, fluorescent protein, luminescent protein
• Biological source: Lobophyllia hemprichii (Lobed brain coral); More
• Total number of polymer chains: 8
• Total formula weight: 100818.04

Authors

Nar, H.,Nienhaus, K.,Wiedenmann, J.,Nienhaus, G.U. (deposition date: 2005-06-01, release date: 2005-06-23, Last modification date: 2023-12-13)

Primary citation

Nienhaus, K.,Nienhaus, G.U.,Wiedenmann, J.,Nar, H.
Structural Basis for Photo-Induced Protein Cleavage and Green-to-Red Conversion of Fluorescent Protein Eosfp.
Proc.Natl.Acad.Sci.USA, 102:9156-, 2005

PubMed Abstract: Genetically encoded fusion constructs derived from fluorescent proteins (FPs) can be designed to report on a multitude of events and signals in cells, tissues, and entire organs without interfering with the complex machinery of life. EosFP is a novel FP from the scleractinian coral Lobophyllia hemprichii that switches its fluorescence emission from green (516 nm) to red (581 nm) upon irradiation with approximately 400-nm light. This property enables localized tagging of proteins and thus provides a valuable tool for tracking protein movements within live cells. Here, we present the x-ray structures of the green and red forms of WT EosFP. They reveal that formation of the red chromophore is associated with cleavage of the peptide backbone, with surprisingly little change elsewhere in the structure, and provide insights into the mechanism that generates this interesting posttranslational polypeptide modification.

PubMed: 15964985
DOI: 10.1073/PNAS.0501874102

Experimental method

X-RAY DIFFRACTION (2 Å)