2BGF

NMR structure of Lys48-linked di-ubiquitin using chemical shift perturbation data together with RDCs and 15N-relaxation data

Summary for 2BGF

• Entry DOI: 10.2210/pdb2bgf/pdb
• Related: 1AAR 1E0Q 1P3Q 1UZX
• Descriptor: DI-UBIQUITIN (1 entity in total)
• Functional Keywords: proteasome, degradation, ubiquitin, polyubiquitin
• Biological source: HOMO SAPIENS (HUMAN)
• Total number of polymer chains: 2
• Total formula weight: 17153.66

Authors

Van Dijk, A.D.J.,Fushman, D.,Bonvin, A.M.J.J. (deposition date: 2004-12-22, release date: 2005-08-31, Last modification date: 2024-05-15)

Primary citation

Van Dijk, A.D.J.,Fushman, D.,Bonvin, A.M.J.J.
Various Strategies of Using Residual Dipolar Couplings in NMR-Driven Protein Docking: Application to Lys48-Linked Di-Ubiquitin and Validation Against 15N-Relaxation Data
Proteins: Struct., Funct., Bioinf., 60:367-, 2005

PubMed Abstract: When classical, Nuclear Overhauser Effect (NOE)-based approaches fail, it is possible, given high-resolution structures of the free molecules, to model the structure of a complex in solution based solely on chemical shift perturbation (CSP) data in combination with orientational restraints from residual dipolar couplings (RDCs) when available. RDCs can be incorporated into the docking following various strategies: as direct restraints and/or as intermolecular intervector projection angle restraints (Meiler et al., J Biomol NMR 2000;16:245-252). The advantage of the latter for docking is that they directly define the relative orientation of the molecules. A combined protocol in which RDCs are first introduced as intervector projection angle restraints and at a later stage as direct restraints is shown here to give the best performance. This approach, implemented in our information-driven docking approach HADDOCK (Dominguez et al., J Am Chem Soc 2003;125:1731-1737), is used to determine the solution structure of the Lys48-linked di-ubiquitin, for which chemical shift mapping, RDCs, and (15)N-relaxation data have been previously obtained (Varadan et al., J Mol Biol 2002;324:637-647). The resulting structures, derived from CSP and RDC data, are cross-validated using (15)N-relaxation data. The solution structure differs from the crystal structure by a 20 degrees rotation of the two ubiquitin units relative to each other.

PubMed: 15937902
DOI: 10.1002/PROT.20476

Experimental method

SOLUTION NMR