252D

CRYSTAL STRUCTURE OF THE B-DNA DECAMER D(CGCAATTGCG)2; SEQUENCE-DEPENDENT CROSSED HELIX PACKING

252D の概要

• エントリーDOI: 10.2210/pdb252d/pdb
• 分子名称: DNA (5'-D(*CP*GP*CP*AP*AP*TP*TP*GP*CP*G)-3') (2 entities in total)
• 機能のキーワード: b-dna, double helix, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 6090.01

構造登録者

Wood, A.A.,Nunn, C.M.,Trent, J.O.,Neidle, S. (登録日: 1996-03-07, 公開日: 1996-04-09, 最終更新日: 2024-02-14)

主引用文献

Wood, A.A.,Nunn, C.M.,Trent, J.O.,Neidle, S.
Sequence-dependent crossed helix packing in the crystal structure of a B-DNA decamer yields a detailed model for the Holliday junction.
J.Mol.Biol., 269:827-841, 1997

PubMed Abstract: The structure of the B-DNA decamer d(CGCAATTGCG)2 has been determined by X-ray diffraction analysis to a resolution of 2.3 A and an R-factor of 17.7%. The decamer crystallises in the monoclinic space group C2 and packs with a crossed arrangement of helices and a unique crossing contact distinct from all other decamer structures. This is believed to be a direct result of the sequence-dependent minor groove width of the duplex. Crossed helix structures of DNA are valuable starting points for modelling studies of the Holliday junction. Two unique sites are observed at the cross-over junction where strand exchange may occur. A Holliday junction model has been constructed for each case and modelled using molecular mechanics and dynamics techniques. One of these models was found to be fully consistent with the available physical data.

PubMed: 9223644
DOI: 10.1006/jmbi.1997.1089

実験手法

X-RAY DIFFRACTION (2.3 Å)