241D

EXTENSION OF THE FOUR-STRANDED INTERCALATED CYTOSINE MOTIF BY ADENINE.ADENINE BASE PAIRING IN THE CRYSTAL STRUCTURE OF D(CCCAAT)

Summary for 241D

• Entry DOI: 10.2210/pdb241d/pdb
• Descriptor: DNA (5'-D(*CP*CP*CP*AP*AP*T)-3') (2 entities in total)
• Functional Keywords: u-dna, quadruple helix, arallel-stranded tetraplex, base intercalated, mismatched, dna
• Total number of polymer chains: 2
• Total formula weight: 3506.39

Authors

Berger, I.,Kang, C.,Fredian, A.,Ratliff, R.,Moyzis, R.,Rich, A. (deposition date: 1995-12-07, release date: 1996-04-03, Last modification date: 2024-02-14)

Primary citation

Berger, I.,Kang, C.,Fredian, A.,Ratliff, R.,Moyzis, R.,Rich, A.
Extension of the four-stranded intercalated cytosine motif by adenine.adenine base pairing in the crystal structure of d(CCCAAT).
Nat.Struct.Biol., 2:416-425, 1995

PubMed Abstract: The crystal structure of d(CCCAAT), refined at 2.0 A resolution, shows a four stranded molecule in which two parallel duplexes intercalate with opposite polarity, using cytosine.protonated cytosine base pairs. The intercalation motif in this structure is extended by adenine.adenine base pairs. Two topologically distinct broad grooves are found in the lath-like central part of the molecule with the phosphate groups on one side bent over towards each other, stabilized by bridging water molecules. At the 3' ends, two arrangements of intermolecular A.A.T base triplets are found, involving both asymmetric and symmetric A.A base pairs joined to thymine residues by Watson-Crick and reverse Hoogsteen base pairing, respectively.

PubMed: 7664099
DOI: 10.1038/nsb0595-416

Experimental method

X-RAY DIFFRACTION (1.85 Å)