1ZZJ

Structure of the third KH domain of hnRNP K in complex with 15-mer ssDNA

1ZZJ の概要

• エントリーDOI: 10.2210/pdb1zzj/pdb
• 関連するPDBエントリー: 1ZZI 1ZZK
• 分子名称: 5'-D(*TP*TP*CP*CP*CP*CP*TP*CP*CP*CP*CP*AP*TP*TP*T)-3', Heterogeneous nuclear ribonucleoprotein K (3 entities in total)
• 機能のキーワード: protein-dna complex, structural protein-dna complex, structural protein/dna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P61978
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 31137.13

構造登録者

Backe, P.H.,Messias, A.C.,Ravelli, R.B.,Sattler, M.,Cusack, S. (登録日: 2005-06-14, 公開日: 2005-08-09, 最終更新日: 2024-03-13)

主引用文献

Backe, P.H.,Messias, A.C.,Ravelli, R.B.,Sattler, M.,Cusack, S.
X-Ray Crystallographic and NMR Studies of the Third KH Domain of hnRNP K in Complex with Single-Stranded Nucleic Acids
STRUCTURE, 13:1055-1067, 2005

PubMed Abstract: The heterogeneous nuclear ribonucleoprotein (hnRNP) K is implicated in multiple functions in the regulation of gene expression and acts as a hub at the intersection of signaling pathways and processes involving nucleic acids. Central to its function is its ability to bind both ssDNA and ssRNA via its KH (hnRNP K homology) domains. We determined crystal structures of hnRNP K KH3 domain complexed with 15-mer and 6-mer (CTC(4)) ssDNAs at 2.4 and 1.8 A resolution, respectively, and show that the KH3 domain binds specifically to both TCCC and CCCC sequences. In parallel, we used NMR to compare the binding affinity and mode of interaction of the KH3 domain with several ssRNA ligands and CTC(4) ssDNA. Based on a structure alignment of the KH3-CTC(4) complex with known structures of other KH domains in complex with ssRNA, we discuss recognition of tetranucleotide sequences by KH domains.

PubMed: 16004877
DOI: 10.1016/j.str.2005.04.008

実験手法

X-RAY DIFFRACTION (2.3 Å)