1ZV1

Crystal structure of the dimerization domain of doublesex protein from D. melanogaster

1ZV1 の概要

• エントリーDOI: 10.2210/pdb1zv1/pdb
• 分子名称: Doublesex protein (2 entities in total)
• 機能のキーワード: doublesex, uba domain, dimerization, sex determination, transcription factor, protein binding
• 由来する生物種: Drosophila melanogaster (fruit fly)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 15491.47

構造登録者

Weiss, M.A.,Bayrer, J.R.,Wan, Z.,Li, B.,Phillips, N.B. (登録日: 2005-06-01, 公開日: 2005-08-09, 最終更新日: 2024-02-14)

主引用文献

Bayrer, J.R.,Zhang, W.,Weiss, M.A.
Dimerization of doublesex is mediated by a cryptic ubiquitin-associated domain fold: implications for sex-specific gene regulation
J.Biol.Chem., 280:32989-32996, 2005

PubMed Abstract: Male- and female-specific isoforms of the Doublesex (DSX) transcription factor regulate somatic sexual differentiation in Drosophila. The isoforms (DSX(M) and DSX(F)) share an N-terminal DNA binding domain (the DM motif), broadly conserved among metazoan sex-determining pathways. DM-DNA recognition is enhanced by a C-terminal dimerization domain. The crystal structure of this domain, determined at a resolution of 1.6 A, reveals a novel dimeric arrangement of ubiquitin-associated (UBA) folds. Although this alpha-helical motif is well characterized in pathways of DNA repair and subcellular trafficking, to our knowledge this is its first report in a transcription factor. Dimerization is mediated by a non-canonical hydrophobic interface extrinsic to the putative ubiquitin binding surface. Key side chains at this interface, identified by alanine scanning mutagenesis, are conserved among DSX homologs. The mechanism of dimerization is thus unrelated to the low affinity domain swapping observed among ubiquitin-associated CUE domains. The unexpected observation of a ubiquitin-associated fold in DSX extends the repertoire of alpha-helical dimerization elements in transcription factors. The possibility that the ubiquitination machinery participates in the regulation of sexual dimorphism is discussed.

PubMed: 16049008
DOI: 10.1074/jbc.M507990200

実験手法

X-RAY DIFFRACTION (1.6 Å)