1ZRL

Crystal structure of EBA-175 Region II (RII)

1ZRL の概要

• エントリーDOI: 10.2210/pdb1zrl/pdb
• 関連するPDBエントリー: 1ZRO
• 分子名称: erythrocyte binding antigen region II, SULFATE ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: eba-175, rii, dbl, erythrocyte, invasion, host, malaria, disease, glycophorin, glycan, sialic acid, cell invasion
• 由来する生物種: Plasmodium falciparum (malaria parasite P. falciparum)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 73084.41

構造登録者

Tolia, N.H.,Enemark, E.J.,Sim, B.K.,Joshua-Tor, L. (登録日: 2005-05-19, 公開日: 2005-08-09, 最終更新日: 2024-11-13)

主引用文献

Tolia, N.H.,Enemark, E.J.,Sim, B.K.,Joshua-Tor, L.
Structural Basis for the EBA-175 Erythrocyte Invasion Pathway of the Malaria Parasite Plasmodium falciparum.
Cell(Cambridge,Mass.), 122:183-193, 2005

PubMed Abstract: Erythrocyte binding antigen 175 (EBA-175) is a P. falciparum protein that binds the major glycoprotein found on human erythrocytes, glycophorin A, during invasion. Here we present the crystal structure of the erythrocyte binding domain of EBA-175, RII, which has been established as a vaccine candidate. Binding sites for the heavily sialylated receptor glycophorin A are proposed based on a complex of RII with a glycan that contains the essential components required for binding. The dimeric organization of RII displays two prominent channels that contain four of the six observed glycan binding sites. Each monomer consists of two Duffy binding-like (DBL) domains (F1 and F2). F2 more prominently lines the channels and makes the majority of the glycan contacts, underscoring its role in cytoadherence and in antigenic variation in malaria. Our studies provide insight into the mechanism of erythrocyte invasion by the malaria parasite and aid in rational drug design and vaccines.

PubMed: 16051144
DOI: 10.1016/j.cell.2005.05.033

実験手法

X-RAY DIFFRACTION (2.3 Å)