1ZR4
Structure of a Synaptic gamma-delta Resolvase Tetramer Covalently linked to two Cleaved DNAs
Summary for 1ZR4
| Entry DOI | 10.2210/pdb1zr4/pdb |
| Related | 1GDR 1GDT 1ZR2 2RSL |
| Descriptor | TCAGTGTCCGATAATTTAT, AAA, TTATCGGACACTG, ... (4 entities in total) |
| Functional Keywords | resolvase, site-specific, recombination, flat interface, cross-crystal averaging, multi-crystal averaging, recombination-dna complex, recombination/dna |
| Biological source | Escherichia coli More |
| Total number of polymer chains | 16 |
| Total formula weight | 124098.08 |
| Authors | Li, W.,Kamtekar, S.,Xiong, Y.,Sarkis, G.J.,Grindley, N.D.,Steitz, T.A. (deposition date: 2005-05-19, release date: 2005-08-30, Last modification date: 2024-11-20) |
| Primary citation | Li, W.,Kamtekar, S.,Xiong, Y.,Sarkis, G.J.,Grindley, N.D.,Steitz, T.A. Structure of a synaptic gamma delta resolvase tetramer covalently linked to two cleaved DNAs. Science, 309:1210-1215, 2005 Cited by PubMed Abstract: The structure of a synaptic intermediate of the site-specific recombinase gammadelta resolvase covalently linked through Ser10 to two cleaved duplex DNAs has been determined at 3.4 angstrom resolution. This resolvase, activated for recombination by mutations, forms a tetramer whose structure is substantially changed from that of a presynaptic complex between dimeric resolvase and the cleavage site DNA. Because the two cleaved DNA duplexes that are to be recombined lie on opposite sides of the core tetramer, large movements of both protein and DNA are required to achieve strand exchange. The two dimers linked to the DNAs that are to be recombined are held together by a flat interface. This may allow a 180 degrees rotation of one dimer relative to the other in order to reposition the DNA duplexes for strand exchange. PubMed: 15994378DOI: 10.1126/science.1112064 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.4 Å) |
Structure validation
Download full validation report
