1ZMM

Crystal structure of human alpha-defensin-4

1ZMM の概要

• エントリーDOI: 10.2210/pdb1zmm/pdb
• 関連するPDBエントリー: 1DFN 1ZMH 1ZMI 1ZMK 1ZMP 1ZMQ
• 分子名称: Neutrophil defensin 4 (2 entities in total)
• 機能のキーワード: human alpha-defensin, antimicrobial peptide, antimicrobial protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P12838
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 14881.94

構造登録者

Lubkowski, J.,Szyk, A.,Lu, W. (登録日: 2005-05-10, 公開日: 2006-05-30, 最終更新日: 2024-10-30)

主引用文献

Szyk, A.,Wu, Z.,Tucker, K.,Yang, D.,Lu, W.,Lubkowski, J.
Crystal structures of human {alpha}-defensins HNP4, HD5, and HD6.
Protein Sci., 15:2749-2760, 2006

PubMed Abstract: Six alpha-defensins have been found in humans. These small arginine-rich peptides play important roles in various processes related to host defense, being the effectors and regulators of innate immunity as well as enhancers of adoptive immune responses. Four defensins, called neutrophil peptides 1 through 4, are stored primarily in polymorphonuclear leukocytes. Major sites of expression of defensins 5 and 6 are Paneth cells of human small intestine. So far, only one structure of human alpha-defensin (HNP3) has been reported, and the properties of the intestine defensins 5 and 6 are particularly poorly understood. In this report, we present the high-resolution X-ray structures of three human defensins, 4 through 6, supplemented with studies of their antimicrobial and chemotactic properties. Despite only modest amino acid sequence identity, all three defensins share their tertiary structures with other known alpha- and beta-defensins. Like HNP3 but in contrast to murine or rabbit alpha-defensins, human defensins 4-6 form characteristic dimers. Whereas antimicrobial and chemotactic activity of HNP4 is somewhat comparable to that of other human neutrophil defensins, neither of the intestinal defensins appears to be chemotactic, and for HD6 also an antimicrobial activity has yet to be observed. The unusual biological inactivity of HD6 may be associated with its structural properties, somewhat standing out when compared with other human alpha-defensins. The strongest cationic properties and unique distribution of charged residues on the molecular surface of HD5 may be associated with its highest bactericidal activity among human alpha-defensins.

PubMed: 17088326
DOI: 10.1110/ps.062336606

実験手法

X-RAY DIFFRACTION (1.6 Å)