1ZME

CRYSTAL STRUCTURE OF PUT3/DNA COMPLEX

1ZME の概要

• エントリーDOI: 10.2210/pdb1zme/pdb
• 分子名称: DNA (5'-D(*AP*CP*GP*GP*GP*AP*AP*GP*CP*CP*AP*AP*CP*TP*CP*CP*G)-3'), DNA (5'-D(*AP*CP*GP*GP*AP*GP*(5IU)P*TP*GP*GP*CP*TP*(5IU)P*CP*CP*CP*G)-3'), PROLINE UTILIZATION TRANSCRIPTION ACTIVATOR, ... (5 entities in total)
• 機能のキーワード: complex (transcription regulation-dna), put3, zn2cys6, binuclear cluster, transcription factor, transcription-dna complex, transcription/dna
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• 細胞内の位置: Nucleus: P25502
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 26837.79

構造登録者

Swaminathan, K.,Marmorstein, R. (登録日: 1997-08-06, 公開日: 1998-09-16, 最終更新日: 2024-02-14)

主引用文献

Swaminathan, K.,Flynn, P.,Reece, R.J.,Marmorstein, R.
Crystal structure of a PUT3-DNA complex reveals a novel mechanism for DNA recognition by a protein containing a Zn2Cys6 binuclear cluster.
Nat.Struct.Biol., 4:751-759, 1997

PubMed Abstract: PUT3 is a member of a family of at least 79 fungal transcription factors that contain a six-cysteine, two-zinc domain called a 'Zn2Cys6 binuclear cluster'. We have determined the crystal structure of the DNA binding region from the PUT3 protein bound to its cognate DNA target. The structure reveals that the PUT3 homodimer is bound asymmetrically to the DNA site. This asymmetry orients a beta-strand from one protein subunit into the minor groove of the DNA resulting in a partial amino acid-base pair intercalation and extensive direct and water-mediated protein interactions with the minor groove of the DNA. These interactions facilitate a sequence dependent kink at the centre of the DNA site and specify the intervening base pairs separating two DNA half-sites that are contacted in the DNA major groove. A comparison with the GAL4-DNA and PPR1-DNA complexes shows how a family of related DNA binding proteins can use a diverse set of mechanisms to discriminate between the base pairs separating conserved DNA half-sites.

PubMed: 9303004
DOI: 10.1038/nsb0997-751

実験手法

X-RAY DIFFRACTION (2.5 Å)