1ZLL

NMR Structure of Unphosphorylated Human Phospholamban Pentamer

1ZLL の概要

• エントリーDOI: 10.2210/pdb1zll/pdb
• 分子名称: Cardiac phospholamban (1 entity in total)
• 機能のキーワード: pentamer, leu/ile zipper, super coil, channel, membrane protein-signaling protein complex, membrane protein/signaling protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Mitochondrion membrane; Single-pass membrane protein: P26678
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 30572.88

構造登録者

Oxenoid, K.,Chou, J.J. (登録日: 2005-05-06, 公開日: 2005-08-09, 最終更新日: 2024-05-22)

主引用文献

Oxenoid, K.,Chou, J.J.
The structure of phospholamban pentamer reveals a channel-like architecture in membranes
Proc.Natl.Acad.Sci.USA, 102:10870-10875, 2005

PubMed Abstract: Contraction and relaxation of heart muscle cells is regulated by cycling of calcium between cytoplasm and sarcoplasmic reticulum. Human phospholamban (PLN), expressed in the sarcoplasmic reticulum membrane as a 30-kDa homopentamer, controls cellular calcium levels by a mechanism that depends on its phosphorylation. Since PLN was discovered approximately 30 years ago, extensive studies have aimed to explain how it influences calcium pumps and to determine whether it acts as an ion channel. We have determined by solution NMR methods the atomic resolution structure of an unphosphorylated PLN pentamer in dodecylphosphocholine micelles. The unusual bellflower-like assembly is held together by leucine/isoleucine zipper motifs along the membrane-spanning helices. The structure reveals a channel-forming architecture that could allow passage of small ions. The central pore gradually widens toward the cytoplasmic end as the transmembrane helices twist around each other and bend outward. The dynamic N-terminal amphipathic helices point away from the membrane, perhaps facilitating recognition and inhibition of the calcium pump.

PubMed: 16043693
DOI: 10.1073/pnas.0504920102

実験手法

SOLUTION NMR