1ZIQ

Deuterated gammaE crystallin in D2O solvent

1ZIQ の概要

• エントリーDOI: 10.2210/pdb1ziq/pdb
• 関連するPDBエントリー: 1A5D 1ZGT 1ZIE 1ZIR
• 分子名称: Gamma crystallin E, ACETATE ION (3 entities in total)
• 機能のキーワード: 4 greek key motifs, structural protein
• 由来する生物種: Rattus norvegicus (Norway rat)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21281.70

構造登録者

Artero, J.B.,Hartlein, M.,McSweeney, S.,Timmins, P. (登録日: 2005-04-27, 公開日: 2005-11-08, 最終更新日: 2023-10-25)

主引用文献

Artero, J.B.,Hartlein, M.,McSweeney, S.,Timmins, P.
A comparison of refined X-ray structures of hydrogenated and perdeuterated rat gammaE-crystallin in H2O and D2O.
Acta Crystallogr.,Sect.D, 61:1541-1549, 2005

PubMed Abstract: Rat gammaE-crystallin was overexpressed, purified under different labelling conditions and crystallized and X-ray data were collected at resolutions between 1.71 and 1.36 A. The structures were determined by molecular replacement. In these structures, the cd loop of the Greek-key motif 3, which is the major structural key motif of the two phase-transition groups of gamma-crystallins, presents a double conformation. The influence of the perdeuteration on the protein structure was determined by comparison of the atomic positions and temperature factors of the different models. The perdeuterated proteins have a similar structure to their hydrogenated counterparts, but partial or full deuteration may have some effect on the atomic B-factor values.

PubMed: 16239733
DOI: 10.1107/S0907444905028532

実験手法

X-RAY DIFFRACTION (1.72 Å)