1ZIG

GAGA RNA TETRALOOP, NMR, 10 STRUCTURES

1ZIG の概要

• エントリーDOI: 10.2210/pdb1zig/pdb
• 関連するPDBエントリー: 1ZIF 1ZIH
• 分子名称: RNA (5'-R(*GP*GP*GP*CP*GP*AP*GP*AP*GP*CP*CP*U)-3') (1 entity in total)
• 機能のキーワード: ribonucleic acid, rna, tetraloops, gnra, hairpin
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3906.40

構造登録者

Jucker, F.M.,Heus, H.A.,Yip, P.F.,Moors, E.,Pardi, A. (登録日: 1996-07-27, 公開日: 1997-03-12, 最終更新日: 2024-05-22)

主引用文献

Jucker, F.M.,Heus, H.A.,Yip, P.F.,Moors, E.H.,Pardi, A.
A network of heterogeneous hydrogen bonds in GNRA tetraloops.
J.Mol.Biol., 264:968-980, 1996

PubMed Abstract: RNA hairpin loops containing a GNRA consensus sequence are the most frequently occurring hairpins in a variety of prokaryotic and eukaryotic RNAs. These tetraloops play important functional roles in RNA folding, in RNA-RNA tertiary interactions and as protein binding sites. Homo and heteronuclear NMR spectroscopy have been used to determine the structures of the most abundant members of the GNRA tetraloop family: the GAGA, GCAA and GAAA loops closed by a C-G base pair. Analysis of the structures of these three hairpin loops reveals a network of heterogeneous hydrogen bonds. The loops contain a G-A base pair, a G base-phosphate hydrogen bond and several 2' OH-base hydrogen bonds. These intramolecular interactions and the extensive base stacking in the loop help explain the high thermodynamic stability and give insight into the diverse biological roles of the GNRA RNA hairpins.

PubMed: 9000624
DOI: 10.1006/jmbi.1996.0690

実験手法

SOLUTION NMR