1ZHO

The structure of a ribosomal protein L1 in complex with mRNA

1ZHO の概要

• エントリーDOI: 10.2210/pdb1zho/pdb
• 関連するPDBエントリー: 1ad2 1CSJ 1DWU 1i2a 1mzp 1U63
• 分子名称: mRNA, 50S ribosomal protein L1, POTASSIUM ION, ... (4 entities in total)
• 機能のキーワード: ribosome, ribosomal protein, mrna-protein complex, l1, regulation of gene expression, rna-protein interaction, structural protein-rna complex, structural protein/rna
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 148746.58

構造登録者

Nevskaya, N.,Tishchenko, S.,Volchkov, S.,Kljashtorny, V.,Nikonova, E.,Nikonov, O.,Nikulin, A.,Kohrer, C.,Piendl, W.,Zimmermann, R.,Stockley, P.,Garber, M.,Nikonov, S. (登録日: 2005-04-26, 公開日: 2006-05-09, 最終更新日: 2024-10-30)

主引用文献

Nevskaya, N.,Tishchenko, S.,Volchkov, S.,Kljashtorny, V.,Nikonova, E.,Nikonov, O.,Nikulin, A.,Kohrer, C.,Piendl, W.,Zimmermann, R.,Stockley, P.,Garber, M.,Nikonov, S.
New insights into the interaction of ribosomal protein L1 with RNA.
J.Mol.Biol., 355:747-759, 2006

PubMed Abstract: The RNA-binding ability of ribosomal protein L1 is of profound interest, since L1 has a dual function as a ribosomal structural protein that binds rRNA and as a translational repressor that binds its own mRNA. Here, we report the crystal structure at 2.6 A resolution of ribosomal protein L1 from the bacterium Thermus thermophilus in complex with a 38 nt fragment of L1 mRNA from Methanoccocus vannielii. The conformation of RNA-bound T.thermophilus L1 differs dramatically from that of the isolated protein. Analysis of four copies of the L1-mRNA complex in the crystal has shown that domain II of the protein does not contribute to mRNA-specific binding. A detailed comparison of the protein-RNA interactions in the L1-mRNA and L1-rRNA complexes identified amino acid residues of L1 crucial for recognition of its specific targets on the both RNAs. Incorporation of the structure of bacterial L1 into a model of the Escherichia coli ribosome revealed two additional contact regions for L1 on the 23S rRNA that were not identified in previous ribosome models.

PubMed: 16330048
DOI: 10.1016/j.jmb.2005.10.084

実験手法

X-RAY DIFFRACTION (2.6 Å)