1ZFU

Plectasin:A peptide antibiotic with therapeutic potential from a saprophytic fungus

1ZFU の概要

• エントリーDOI: 10.2210/pdb1zfu/pdb
• 分子名称: Plectasin (1 entity in total)
• 機能のキーワード: defensin, antimicrobial peptide, cysteine stabilized alpha-beta motif, antimicrobial protein
• 由来する生物種: Pseudoplectania nigrella
• 細胞内の位置: Secreted (Probable): Q53I06
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4415.02

構造登録者

Mygind, P.H.,Fischer, R.L.,Schnorr, K.,Hansen, M.T.,Sonksen, C.P.,Ludvigsen, S.,Raventos, D.,Buskov, S.,Christensen, B.,De Maria, L.,Taboureau, O.,Yaver, D.,Elvig-Jorgensen, S.G.,Sorensen, M.V.,Christensen, B.E.,Kjaerulf, S.,Frimodt-Moller, N.,Lehrer, R.I.,Zasloff, M.,Kristensen, H.H. (登録日: 2005-04-20, 公開日: 2005-10-18, 最終更新日: 2024-10-30)

主引用文献

Mygind, P.H.,Fischer, R.L.,Schnorr, K.M.,Hansen, M.T.,Sonksen, C.P.,Ludvigsen, S.,Raventos, D.,Buskov, S.,Christensen, B.,De Maria, L.,Taboureau, O.,Yaver, D.,Elvig-Jorgensen, S.G.,Sorensen, M.V.,Christensen, B.E.,Kjaerulff, S.,Frimodt-Moller, N.,Lehrer, R.I.,Zasloff, M.,Kristensen, H.H.
Plectasin is a peptide antibiotic with therapeutic potential from a saprophytic fungus.
Nature, 437:975-980, 2005

PubMed Abstract: Animals and higher plants express endogenous peptide antibiotics called defensins. These small cysteine-rich peptides are active against bacteria, fungi and viruses. Here we describe plectasin-the first defensin to be isolated from a fungus, the saprophytic ascomycete Pseudoplectania nigrella. Plectasin has primary, secondary and tertiary structures that closely resemble those of defensins found in spiders, scorpions, dragonflies and mussels. Recombinant plectasin was produced at a very high, and commercially viable, yield and purity. In vitro, the recombinant peptide was especially active against Streptococcus pneumoniae, including strains resistant to conventional antibiotics. Plectasin showed extremely low toxicity in mice, and cured them of experimental peritonitis and pneumonia caused by S. pneumoniae as efficaciously as vancomycin and penicillin. These findings identify fungi as a novel source of antimicrobial defensins, and show the therapeutic potential of plectasin. They also suggest that the defensins of insects, molluscs and fungi arose from a common ancestral gene.

PubMed: 16222292
DOI: 10.1038/nature04051

実験手法

SOLUTION NMR