1ZE2

Conformational change of pseudouridine 55 synthase upon its association with RNA substrate

1ZE2 の概要

• エントリーDOI: 10.2210/pdb1ze2/pdb
• 関連するPDBエントリー: 1ZE1
• 分子名称: 5'-R(*GP*GP*CP*CP*AP*CP*GP*GP*UP*(FHU)P*CP*GP*AP*AP*UP*CP*CP*GP*UP*GP*GP*C)-3', tRNA pseudouridine synthase B (3 entities in total)
• 機能のキーワード: protein-rna complex, lyase-rna complex, lyase/rna
• 由来する生物種: Thermotoga maritima
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 85290.92

構造登録者

Phannachet, K.,Huang, R.H. (登録日: 2005-04-16, 公開日: 2005-04-26, 最終更新日: 2024-02-14)

主引用文献

Phannachet, K.,Huang, R.H.
Conformational change of pseudouridine 55 synthase upon its association with RNA substrate
Nucleic Acids Res., 32:1422-1429, 2004

PubMed Abstract: Pseudouridine 55 synthase (Psi55S) catalyzes isomerization of uridine (U) to pseudouridine (Psi) at position 55 in transfer RNA. The crystal structures of Thermotoga maritima Psi55S, and its complex with RNA, have been determined at 2.9 and 3.0 A resolutions, respectively. Structural comparisons with other families of pseudouridine synthases (PsiS) indicate that Psi55S may acquire its ability to recognize a stem-loop RNA substrate by two insertions of polypeptides into the PsiS core. The structure of apo-Psi55S reveals that these two insertions interact with each other. However, association with RNA substrate induces substantial conformational change in one of the insertions, resulting in disruption of interaction between insertions and association of both insertions with the RNA substrate. Specific interactions between two insertions, as well as between the insertions and the RNA substrate, account for the molecular basis of the conformational change.

PubMed: 14990747
DOI: 10.1093/nar/gkh287

実験手法

X-RAY DIFFRACTION (3 Å)