1ZB1

Structure basis for endosomal targeting by the Bro1 domain

1ZB1 の概要

• エントリーDOI: 10.2210/pdb1zb1/pdb
• 分子名称: BRO1 protein (2 entities in total)
• 機能のキーワード: bro1, aip1, bro1 domain, snf7, trafficking, protein transport
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Cytoplasm: P48582
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 91215.16

構造登録者

Kim, J.,Sitaraman, S.,Hierro, A.,Beach, B.M.,Odorizzi, G.,Hurley, J.H. (登録日: 2005-04-07, 公開日: 2005-06-14, 最終更新日: 2024-02-14)

主引用文献

Kim, J.,Sitaraman, S.,Hierro, A.,Beach, B.M.,Odorizzi, G.,Hurley, J.H.
Structural basis for endosomal targeting by the Bro1 domain.
Dev.Cell, 8:937-947, 2005

PubMed Abstract: Proteins delivered to the lysosome or the yeast vacuole via late endosomes are sorted by the ESCRT complexes and by associated proteins, including Alix and its yeast homolog Bro1. Alix, Bro1, and several other late endosomal proteins share a conserved 160 residue Bro1 domain whose boundaries, structure, and function have not been characterized. The crystal structure of the Bro1 domain of Bro1 reveals a folded core of 367 residues. The extended Bro1 domain is necessary and sufficient for binding to the ESCRT-III subunit Snf7 and for the recruitment of Bro1 to late endosomes. The structure resembles a boomerang with its concave face filled in and contains a triple tetratricopeptide repeat domain as a substructure. Snf7 binds to a conserved hydrophobic patch on Bro1 that is required for protein complex formation and for the protein-sorting function of Bro1. These results define a conserved mechanism whereby Bro1 domain-containing proteins are targeted to endosomes by Snf7 and its orthologs.

PubMed: 15935782
DOI: 10.1016/j.devcel.2005.04.001

実験手法

X-RAY DIFFRACTION (1.95 Å)