1Z7Z

Cryo-em structure of human coxsackievirus A21 complexed with five domain icam-1kilifi

Summary for 1Z7Z

• Entry DOI: 10.2210/pdb1z7z/pdb
• Related: 1IAM 1Z7S
• EMDB information: 1114
• Descriptor: human coxsackievirus A21, Intercellular adhesion molecule-1, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• Functional Keywords: icam-1, kilifi, cd54, human coxsackievirus a21, virus-receptor complex, icosahedral virus, virus/receptor
• Biological source: Human coxsackievirus A21; More
• Total number of polymer chains: 6
• Total formula weight: 140661.67

Authors

Xiao, C.,Bator-Kelly, C.M.,Rieder, E.,Chipman, P.R.,Craig, A.,Kuhn, R.J.,Wimmer, E.,Rossmann, M.G. (deposition date: 2005-03-28, release date: 2005-08-02, Last modification date: 2021-10-20)

Primary citation

Xiao, C.,Bator-Kelly, C.M.,Rieder, E.,Chipman, P.R.,Craig, A.,Kuhn, R.J.,Wimmer, E.,Rossmann, M.G.
The crystal structure of coxsackievirus a21 and its interaction with icam-1.
Structure, 13:1019-1033, 2005

PubMed Abstract: CVA21 and polioviruses both belong to the Enterovirus genus in the family of Picornaviridae, whereas rhinoviruses form a distinct picornavirus genus. Nevertheless, CVA21 and the major group of human rhinoviruses recognize intercellular adhesion molecule-1 (ICAM-1) as their cellular receptor, whereas polioviruses use poliovirus receptor. The crystal structure of CVA21 has been determined to 3.2 A resolution. Its structure has greater similarity to poliovirus structures than to other known picornavirus structures. Cryo-electron microscopy (cryo-EM) was used to determine an 8.0 A resolution structure of CVA21 complexed with an ICAM-1 variant, ICAM-1(Kilifi). The cryo-EM map was fitted with the crystal structures of ICAM-1 and CVA21. Significant differences in the structure of CVA21 with respect to the poliovirus structures account for the inability of ICAM-1 to bind polioviruses. The interface between CVA21 and ICAM-1 has shape and electrostatic complementarity with many residues being conserved among those CVAs that bind ICAM-1.

PubMed: 16004874
DOI: 10.1016/j.str.2005.04.011

Experimental method

ELECTRON MICROSCOPY (8 Å)