1Z7M

ATP Phosphoribosyl transferase (HisZG ATP-PRTase) from Lactococcus lactis

1Z7M の概要

• エントリーDOI: 10.2210/pdb1z7m/pdb
• 関連するPDBエントリー: 1Z7N
• 分子名称: ATP phosphoribosyltransferase regulatory subunit, ATP phosphoribosyltransferase, TUNGSTATE(VI)ION, ... (4 entities in total)
• 機能のキーワード: atp-prt, histidine biosynthesis, transferase, hiszg, allosteric, evolution
• 由来する生物種: Lactococcus lactis; 詳細
• 細胞内の位置: Cytoplasm (Probable): Q02147; Cytoplasm (By similarity): Q02129
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 255292.30

構造登録者

Champagne, K.S.,Sissler, M.,Larrabee, Y.,Doublie, S.,Francklyn, C.S. (登録日: 2005-03-25, 公開日: 2005-08-09, 最終更新日: 2024-02-14)

主引用文献

Champagne, K.S.,Sissler, M.,Larrabee, Y.,Doublie, S.,Francklyn, C.S.
Activation of the hetero-octameric ATP phosphoribosyl transferase through subunit interface rearrangement by a tRNA synthetase paralog.
J.Biol.Chem., 280:34096-34104, 2005

PubMed Abstract: ATP phosphoribosyl transferase (ATP-PRT) joins ATP and 5-phosphoribosyl-1-pyrophosphate (PRPP) in a highly regulated reaction that initiates histidine biosynthesis. The unusual hetero-octameric version of ATP-PRT includes four HisG(S) catalytic subunits based on the periplasmic binding protein fold and four HisZ regulatory subunits that resemble histidyl-tRNA synthetases. Here, we present the first structure of a PRPP-bound ATP-PRT at 2.9 A and provide a structural model for allosteric activation based on comparisons with other inhibited and activated ATP-PRTs from both the hetero-octameric and hexameric families. The activated state of the octameric enzyme is characterized by an interstitial phosphate ion in the HisZ-HisG interface and new contacts between the HisZ motif 2 loop and the HisG(S) dimer interface. These contacts restructure the interface to recruit conserved residues to the active site, where they activate pyrophosphate to promote catalysis. Additionally, mutational analysis identifies the histidine binding sites within a region highly conserved between HisZ and the functional HisRS. Through the oligomerization and functional re-assignment of protein domains associated with aminoacylation and phosphate binding, the HisZ-HisG octameric ATP-PRT acquired the ability to initiate the synthesis of a key metabolic intermediate in an allosterically regulated fashion.

PubMed: 16051603
DOI: 10.1074/jbc.M505041200

実験手法

X-RAY DIFFRACTION (2.9 Å)