1Z77

Crystal structure of transcriptional regulator protein from Thermotoga maritima.

1Z77 の概要

• エントリーDOI: 10.2210/pdb1z77/pdb
• 分子名称: transcriptional regulator (TetR family), 1,2-ETHANEDIOL (3 entities in total)
• 機能のキーワード: transcriptional regulator, tetr family, structural genomics, psi, protein structure initiative, midwest center for structural genomics, mcsg, transcription
• 由来する生物種: Thermotoga maritima
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24338.01

構造登録者

Koclega, K.D.,Chruszcz, M.,Zimmerman, M.D.,Cymborowski, M.,Kudritska, M.,Minor, W.,Midwest Center for Structural Genomics (MCSG) (登録日: 2005-03-24, 公開日: 2005-05-10, 最終更新日: 2024-11-20)

主引用文献

Koclega, K.D.,Chruszcz, M.,Zimmerman, M.D.,Cymborowski, M.,Evdokimova, E.,Minor, W.
Crystal structure of a transcriptional regulator TM1030 from Thermotoga maritima solved by an unusual MAD experiment.
J.Struct.Biol., 159:424-432, 2007

PubMed Abstract: The crystal structure of a putative transcriptional regulator protein TM1030 from Thermotoga maritima, a hyperthermophilic bacterium, was determined by an unusual multi-wavelength anomalous dispersion method at 2.0 A resolution, in which data from two different crystals and two different beamlines were used. The protein belongs to the tetracycline repressor TetR superfamily. The three-dimensional structure of TM1030 is similar to the structures of proteins that function as multidrug-binding transcriptional repressors, and contains a large solvent-exposed pocket similar to the drug-binding pockets present in those repressors. The asymmetric unit in the crystal structure contains a single protein chain and the twofold symmetry of the dimer is adopted by the crystal symmetry. The structure described in this paper is an apo- form of TM1030. Although it is known that the protein is significantly overexpressed during heat shock, its detailed function cannot be yet explained.

PubMed: 17588774
DOI: 10.1016/j.jsb.2007.04.012

実験手法

X-RAY DIFFRACTION (2 Å)