1YVR

Ro autoantigen

1YVR の概要

• エントリーDOI: 10.2210/pdb1yvr/pdb
• 関連するPDBエントリー: 1YVP
• 分子名称: 60-kDa SS-A/Ro ribonucleoprotein (2 entities in total)
• 機能のキーワード: heat repeat; von willebrand factor a; rossmann fold; midas motif', rna binding protein
• 由来する生物種: Xenopus laevis (African clawed frog)
• 細胞内の位置: Cytoplasm: P42700
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 60772.38

構造登録者

Stein, A.J.,Fuchs, G.,Fu, C.,Wolin, S.L.,Reinisch, K.M. (登録日: 2005-02-16, 公開日: 2005-06-07, 最終更新日: 2024-02-14)

主引用文献

Stein, A.J.,Fuchs, G.,Fu, C.,Wolin, S.L.,Reinisch, K.M.
Structural Insights into RNA Quality Control: The Ro Autoantigen Binds Misfolded RNAs via Its Central Cavity
Cell(Cambridge,Mass.), 121:529-539, 2005

PubMed Abstract: The Ro 60 kDa autoantigen is a major target of the immune response in patients with systemic lupus erythematosus. In vertebrate cells, Ro binds misfolded small RNAs and likely functions in RNA quality control. In eukaryotes and bacteria, Ro also associates with small RNAs called Y RNAs. We present structures of unliganded Ro and Ro complexed with two RNAs at 1.95 and 2.2 A resolution, respectively. Ro consists of a von Willebrand factor A domain and a doughnut-shaped domain composed of HEAT repeats. In the complex, a fragment of Y RNA binds on the outer surface of the HEAT-repeat ring, and single-stranded RNA binds in the toroid hole. Mutagenesis supports a binding site for misfolded RNAs that encompasses both sites, with a single-stranded end inserted into the toroid cavity. Our experiments suggest that one role of Y RNAs may be to regulate access of other RNAs to Ro.

PubMed: 15907467
DOI: 10.1016/j.cell.2005.03.009

実験手法

X-RAY DIFFRACTION (1.95 Å)