1YU2

Major Tropism Determinant M1 Variant

Summary for 1YU2

• Entry DOI: 10.2210/pdb1yu2/pdb
• Related: 1YU0 1YU1 1YU3 1YU4
• Descriptor: Major Tropism Determinant (Mtd-M1), MAGNESIUM ION (3 entities in total)
• Functional Keywords: c-type lectin, beta sandwich, beta prism, variability, diversity-generating retroelement, viral protein
• Biological source: Bordetella phage BMP-1
• Total number of polymer chains: 1
• Total formula weight: 39628.70

Authors

McMahon, S.A.,Miller, J.L.,Lawton, J.A.,Ghosh, P. (deposition date: 2005-02-11, release date: 2005-09-20, Last modification date: 2023-08-23)

Primary citation

McMahon, S.A.,Miller, J.L.,Lawton, J.A.,Kerkow, D.E.,Hodes, A.,Marti-Renom, M.A.,Doulatov, S.,Narayanan, E.,Sali, A.,Miller, J.F.,Ghosh, P.
The C-type lectin fold as an evolutionary solution for massive sequence variation
Nat.Struct.Mol.Biol., 12:886-892, 2005

PubMed Abstract: Only few instances are known of protein folds that tolerate massive sequence variation for the sake of binding diversity. The most extensively characterized is the immunoglobulin fold. We now add to this the C-type lectin (CLec) fold, as found in the major tropism determinant (Mtd), a retroelement-encoded receptor-binding protein of Bordetella bacteriophage. Variation in Mtd, with its approximately 10(13) possible sequences, enables phage adaptation to Bordetella spp. Mtd is an intertwined, pyramid-shaped trimer, with variable residues organized by its CLec fold into discrete receptor-binding sites. The CLec fold provides a highly static scaffold for combinatorial display of variable residues, probably reflecting a different evolutionary solution for balancing diversity against stability from that in the immunoglobulin fold. Mtd variants are biased toward the receptor pertactin, and there is evidence that the CLec fold is used broadly for sequence variation by related retroelements.

PubMed: 16170324
DOI: 10.1038/nsmb992

Experimental method

X-RAY DIFFRACTION (1.86 Å)