1YU2

Major Tropism Determinant M1 Variant

1YU2 の概要

• エントリーDOI: 10.2210/pdb1yu2/pdb
• 関連するPDBエントリー: 1YU0 1YU1 1YU3 1YU4
• 分子名称: Major Tropism Determinant (Mtd-M1), MAGNESIUM ION (3 entities in total)
• 機能のキーワード: c-type lectin, beta sandwich, beta prism, variability, diversity-generating retroelement, viral protein
• 由来する生物種: Bordetella phage BMP-1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39628.70

構造登録者

McMahon, S.A.,Miller, J.L.,Lawton, J.A.,Ghosh, P. (登録日: 2005-02-11, 公開日: 2005-09-20, 最終更新日: 2023-08-23)

主引用文献

McMahon, S.A.,Miller, J.L.,Lawton, J.A.,Kerkow, D.E.,Hodes, A.,Marti-Renom, M.A.,Doulatov, S.,Narayanan, E.,Sali, A.,Miller, J.F.,Ghosh, P.
The C-type lectin fold as an evolutionary solution for massive sequence variation
Nat.Struct.Mol.Biol., 12:886-892, 2005

PubMed Abstract: Only few instances are known of protein folds that tolerate massive sequence variation for the sake of binding diversity. The most extensively characterized is the immunoglobulin fold. We now add to this the C-type lectin (CLec) fold, as found in the major tropism determinant (Mtd), a retroelement-encoded receptor-binding protein of Bordetella bacteriophage. Variation in Mtd, with its approximately 10(13) possible sequences, enables phage adaptation to Bordetella spp. Mtd is an intertwined, pyramid-shaped trimer, with variable residues organized by its CLec fold into discrete receptor-binding sites. The CLec fold provides a highly static scaffold for combinatorial display of variable residues, probably reflecting a different evolutionary solution for balancing diversity against stability from that in the immunoglobulin fold. Mtd variants are biased toward the receptor pertactin, and there is evidence that the CLec fold is used broadly for sequence variation by related retroelements.

PubMed: 16170324
DOI: 10.1038/nsmb992

実験手法

X-RAY DIFFRACTION (1.86 Å)