1YSB

Yeast Cytosine Deaminase Triple Mutant

1YSB の概要

• エントリーDOI: 10.2210/pdb1ysb/pdb
• 関連するPDBエントリー: 1YSD
• 分子名称: Cytosine deaminase, ZINC ION, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 35936.38

構造登録者

Korkegian, A.,Black, M.E.,Baker, D.,Stoddard, B.L. (登録日: 2005-02-08, 公開日: 2005-05-17, 最終更新日: 2024-02-14)

主引用文献

Korkegian, A.,Black, M.E.,Baker, D.,Stoddard, B.L.
Computational thermostabilization of an enzyme.
Science, 308:857-860, 2005

PubMed Abstract: Thermostabilizing an enzyme while maintaining its activity for industrial or biomedical applications can be difficult with traditional selection methods. We describe a rapid computational approach that identified three mutations within a model enzyme that produced a 10 degrees C increase in apparent melting temperature T(m) and a 30-fold increase in half-life at 50 degrees C, with no reduction in catalytic efficiency. The effects of the mutations were synergistic, giving an increase in excess of the sum of their individual effects. The redesigned enzyme induced an increased, temperature-dependent bacterial growth rate under conditions that required its activity, thereby coupling molecular and metabolic engineering.

PubMed: 15879217
DOI: 10.1126/science.1107387

実験手法

X-RAY DIFFRACTION (1.7 Å)