1YLB

NMR solution structure of the reduced spinach plastocyanin

1YLB の概要

• エントリーDOI: 10.2210/pdb1ylb/pdb
• 分子名称: Plastocyanin, chloroplast, COPPER (I) ION (2 entities in total)
• 機能のキーワード: plastocyanin, copper(+)-containing, electron-transfer, spinach, photosynthesis, blue-copper protein, electron transport
• 由来する生物種: Spinacia oleracea (spinach)
• 細胞内の位置: Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Lumenal side: P00289
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10484.10

構造登録者

Musiani, F.,Dikiy, A.,Semenov, A.Y.,Ciurli, S. (登録日: 2005-01-19, 公開日: 2005-04-05, 最終更新日: 2024-05-22)

主引用文献

Musiani, F.,Dikiy, A.,Semenov, A.Y.,Ciurli, S.
Structure of the Intermolecular Complex between Plastocyanin and Cytochrome f from Spinach.
J.Biol.Chem., 280:18833-18841, 2005

PubMed Abstract: In oxygenic photosynthesis, plastocyanin shuttles electrons between the membrane-bound complexes cytochrome b6f and photosystem I. The homologous complex between cytochrome f and plastocyanin, both from spinach, is the object of this study. The solution structure of the reduced spinach plastocyanin was determined using high field NMR spectroscopy, whereas the model structure of oxidized cytochrome f was obtained by homology modeling calculations and molecular dynamics. The model structure of the intermolecular complex was calculated using the program AUTODOCK, taking into account biological information obtained from mutagenesis experiments. The best electron transfer pathway from the heme group of cytochrome f to the copper ion of plastocyanin was calculated using the program HARLEM, obtaining a coupling decay value of 1.8 x 10(-4). Possible mechanisms of interaction and electron transfer between plastocyanin and cytochrome f were discussed considering the possible formation of a supercomplex that associates one cytochrome b6f, one photosystem I, and one plastocyanin.

PubMed: 15691836
DOI: 10.1074/jbc.M412760200

実験手法

SOLUTION NMR