1YKS

Crystal structure of yellow fever virus NS3 helicase

1YKS の概要

• エントリーDOI: 10.2210/pdb1yks/pdb
• 関連するPDBエントリー: 1YMF
• 分子名称: Genome polyprotein [contains: Flavivirin protease NS3 catalytic subunit] (2 entities in total)
• 機能のキーワード: helicase, flavivirus, dead-box, atpase, rtpase, yellow fever virus, hydrolase
• 由来する生物種: Yellow fever virus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 49400.46

構造登録者

Wu, J.,Bera, A.K.,Kuhn, R.J.,Smith, J.L. (登録日: 2005-01-18, 公開日: 2005-08-23, 最終更新日: 2024-02-14)

主引用文献

Wu, J.,Bera, A.K.,Kuhn, R.J.,Smith, J.L.
Structure of the flavivirus helicase: implications for catalytic activity, protein interactions, and proteolytic processing.
J.Virol., 79:10268-10277, 2005

PubMed Abstract: Yellow fever virus (YFV), a member of the Flavivirus genus, has a plus-sense RNA genome encoding a single polyprotein. Viral protein NS3 includes a protease and a helicase that are essential to virus replication and to RNA capping. The 1.8-A crystal structure of the helicase region of the YFV NS3 protein includes residues 187 to 623. Two familiar helicase domains bind nucleotide in a triphosphate pocket without base recognition, providing a site for nonspecific hydrolysis of nucleoside triphosphates and RNA triphosphate. The third, C-terminal domain has a unique structure and is proposed to function in RNA and protein recognition. The organization of the three domains indicates that cleavage of the viral polyprotein NS3-NS4A junction occurs in trans.

PubMed: 16051820
DOI: 10.1128/JVI.79.16.10268-10277.2005

実験手法

X-RAY DIFFRACTION (1.8 Å)