1YI8

Crystal structure of tryptophanyl trRNA synthetase II from Deinococcus radiodurans in complex with L-Trp

Summary for 1YI8

Entry DOI	10.2210/pdb1yi8/pdb
Descriptor	tryptophanyl-tRNA synthetase, TRYPTOPHAN (3 entities in total)
Functional Keywords	auxiliary tryptophanyl trna synthetase, ligase
Biological source	Deinococcus radiodurans
Total number of polymer chains	3
Total formula weight	114880.98
Authors	Buddha, M.R.,Crane, B.R. (deposition date: 2005-01-11, release date: 2005-02-22, Last modification date: 2024-02-14)
Primary citation	Buddha, M.R.,Crane, B.R. Structure and activity of an aminoacyl-tRNA synthetase that charges tRNA with nitro-tryptophan. Nat.Struct.Mol.Biol., 12:274-275, 2005 Cited by PubMed Abstract: The most divergent of two tryptophanyl tRNA synthetases (TrpRS II) found in Deinococcus radiodurans interacts with a nitric oxide synthase protein that produces 4-nitro-tryptophan (4-NRP). TrpRS II efficiently charges transfer RNA(Trp) with 4-NRP and 5-hydroxy-tryptophan (5-HRP). The crystal structures of TrpRS II bound to tryptophan and 5-HRP reveal residue substitutions that accommodate modified indoles. A class of auxiliary bacterial TrpRSs conserve this capacity to charge tRNA with nonstandard amino acids. PubMed: 15723076 DOI: 10.1038/nsmb907 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.1 Å)

Structure validation