1YI8

Crystal structure of tryptophanyl trRNA synthetase II from Deinococcus radiodurans in complex with L-Trp

1YI8 の概要

• エントリーDOI: 10.2210/pdb1yi8/pdb
• 分子名称: tryptophanyl-tRNA synthetase, TRYPTOPHAN (3 entities in total)
• 機能のキーワード: auxiliary tryptophanyl trna synthetase, ligase
• 由来する生物種: Deinococcus radiodurans
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 114880.98

構造登録者

Buddha, M.R.,Crane, B.R. (登録日: 2005-01-11, 公開日: 2005-02-22, 最終更新日: 2024-02-14)

主引用文献

Buddha, M.R.,Crane, B.R.
Structure and activity of an aminoacyl-tRNA synthetase that charges tRNA with nitro-tryptophan.
Nat.Struct.Mol.Biol., 12:274-275, 2005

PubMed Abstract: The most divergent of two tryptophanyl tRNA synthetases (TrpRS II) found in Deinococcus radiodurans interacts with a nitric oxide synthase protein that produces 4-nitro-tryptophan (4-NRP). TrpRS II efficiently charges transfer RNA(Trp) with 4-NRP and 5-hydroxy-tryptophan (5-HRP). The crystal structures of TrpRS II bound to tryptophan and 5-HRP reveal residue substitutions that accommodate modified indoles. A class of auxiliary bacterial TrpRSs conserve this capacity to charge tRNA with nonstandard amino acids.

PubMed: 15723076
DOI: 10.1038/nsmb907

実験手法

X-RAY DIFFRACTION (2.1 Å)