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1YF2

Three-dimensional structure of DNA sequence specificity (S) subunit of a type I restriction-modification enzyme and its functional implications

Summary for 1YF2
Entry DOI10.2210/pdb1yf2/pdb
DescriptorType I restriction-modification enzyme, S subunit (2 entities in total)
Functional Keywordstype i restriction modification enzyme, s-subunit, structural genomics, psi, protein structure initiative, berkeley structural genomics center, bsgc, hydrolase regulator
Biological sourceMethanocaldococcus jannaschii
Total number of polymer chains2
Total formula weight97279.11
Authors
Kim, J.S.,Degiovanni, A.,Jancarik, J.,Adams, P.D.,Yokota, H.A.,Kim, R.,Kim, S.H.,Berkeley Structural Genomics Center (BSGC) (deposition date: 2004-12-30, release date: 2005-02-15, Last modification date: 2024-02-14)
Primary citationKim, J.S.,Degiovanni, A.,Jancarik, J.,Adams, P.D.,Yokota, H.,Kim, R.,Kim, S.H.
Crystal structure of DNA sequence specificity subunit of a type I restriction-modification enzyme and its functional implications.
Proc.Natl.Acad.Sci.USA, 102:3248-3253, 2005
Cited by
PubMed Abstract: Type I restriction-modification enzymes are differentiated from type II and type III enzymes by their recognition of two specific dsDNA sequences separated by a given spacer and cleaving DNA randomly away from the recognition sites. They are oligomeric proteins formed by three subunits: a specificity subunit, a methylation subunit, and a restriction subunit. We solved the crystal structure of a specificity subunit from Methanococcus jannaschii at 2.4-A resolution. Two highly conserved regions (CRs) in the middle and at the C terminus form a coiled-coil of long antiparallel alpha-helices. Two target recognition domains form globular structures with almost identical topologies and two separate DNA binding clefts with a modeled DNA helix axis positioned across the CR helices. The structure suggests that the coiled-coil CRs act as a molecular ruler for the separation between two recognized DNA sequences. Furthermore, the relative orientation of the two DNA binding clefts suggests kinking of bound dsDNA and exposing of target adenines from the recognized DNA sequences.
PubMed: 15728358
DOI: 10.1073/pnas.0409851102
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.4 Å)
Structure validation

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数据于2025-07-16公开中

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