1YCR

MDM2 BOUND TO THE TRANSACTIVATION DOMAIN OF P53

1YCR の概要

• エントリーDOI: 10.2210/pdb1ycr/pdb
• 分子名称: MDM2, P53 (2 entities in total)
• 機能のキーワード: anti-oncogene, dna-binding, transcription regulation, nuclear protein, complex (oncogene protein-peptide), phosphorylation, activator, complex (oncogene protein-peptide) complex, complex (oncogene protein/peptide)
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 14343.32

構造登録者

Kussie, P.H.,Pavletich, N.P. (登録日: 1996-09-30, 公開日: 1997-11-19, 最終更新日: 2024-02-14)

主引用文献

Kussie, P.H.,Gorina, S.,Marechal, V.,Elenbaas, B.,Moreau, J.,Levine, A.J.,Pavletich, N.P.
Structure of the MDM2 oncoprotein bound to the p53 tumor suppressor transactivation domain.
Science, 274:948-953, 1996

PubMed Abstract: The MDM2 oncoprotein is a cellular inhibitor of the p53 tumor suppressor in that it can bind the transactivation domain of p53 and downregulate its ability to activate transcription. In certain cancers, MDM2 amplification is a common event and contributes to the inactivation of p53. The crystal structure of the 109-residue amino-terminal domain of MDM2 bound to a 15-residue transactivation domain peptide of p53 revealed that MDM2 has a deep hydrophobic cleft on which the p53 peptide binds as an amphipathic alpha helix. The interface relies on the steric complementarity between the MDM2 cleft and the hydrophobic face of the p53 alpha helix and, in particular, on a triad of p53 amino acids-Phe19, Trp23, and Leu26-which insert deep into the MDM2 cleft. These same p53 residues are also involved in transactivation, supporting the hypothesis that MDM2 inactivates p53 by concealing its transactivation domain. The structure also suggests that the amphipathic alpha helix may be a common structural motif in the binding of a diverse family of transactivation factors to the TATA-binding protein-associated factors.

PubMed: 8875929
DOI: 10.1126/science.274.5289.948

実験手法

X-RAY DIFFRACTION (2.6 Å)