1Y7V

X-ray structure of human acid-beta-glucosidase covalently bound to conduritol B epoxide

1Y7V の概要

• エントリーDOI: 10.2210/pdb1y7v/pdb
• 関連するPDBエントリー: 1OGS
• 分子名称: Glucosylceramidase, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: gaucher disease, glucosidase, glucocerebrosidase, cerezyme, hydrolase, glycosidase, sphingolipid metabolism, glycoprotein, lysosome, membrane, disease mutation, alternative initiation, israel structural proteomics center, ispc, structural genomics
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 113738.27

構造登録者

Premkumar, L.,Sawkar, A.R.,Boldin-Adamsky, S.,Toker, L.,Silman, I.,Kelly, J.W.,Futerman, A.H.,Sussman, J.L.,Israel Structural Proteomics Center (ISPC) (登録日: 2004-12-10, 公開日: 2005-04-12, 最終更新日: 2024-10-16)

主引用文献

Premkumar, L.,Sawkar, A.R.,Boldin-Adamsky, S.,Toker, L.,Silman, I.,Kelly, J.W.,Futerman, A.H.,Sussman, J.L.
X-ray structure of human acid-beta-glucosidase covalently bound to conduritol-B-epoxide. Implications for Gaucher disease.
J.Biol.Chem., 280:23815-23819, 2005

PubMed Abstract: Gaucher disease is an inherited metabolic disorder caused by mutations in the lysosomal enzyme acid-beta-glucosidase (GlcCerase). We recently determined the x-ray structure of GlcCerase to 2.0 A resolution (Dvir, H., Harel, M., McCarthy, A. A., Toker, L., Silman, I., Futerman, A. H., and Sussman, J. L. (2003) EMBO Rep.4, 704-709) and have now solved the structure of Glc-Cerase conjugated with an irreversible inhibitor, conduritol-B-epoxide (CBE). The crystal structure reveals that binding of CBE to the active site does not induce a global conformational change in GlcCerase and confirms that Glu340 is the catalytic nucleophile. However, only one of two alternative conformations of a pair of flexible loops (residues 345-349 and 394-399) located at the entrance to the active site in native GlcCerase is observed in the GlcCerase-CBE structure, a conformation in which the active site is accessible to CBE. Analysis of the dynamics of these two alternative conformations suggests that the two loops act as a lid at the entrance to the active site. This possibility is supported by a cluster of mutations in loop 394-399 that cause Gaucher disease by reducing catalytic activity. Moreover, in silico mutational analysis demonstrates that all these mutations stabilize the conformation that limits access to the active site, thus providing a mechanistic explanation of how mutations in this loop result in Gaucher disease.

PubMed: 15817452
DOI: 10.1074/jbc.M502799200

実験手法

X-RAY DIFFRACTION (2.4 Å)