1Y2A

Structure of mammalian importin bound to the non-classical PLSCR1-NLS

1Y2A の概要

• エントリーDOI: 10.2210/pdb1y2a/pdb
• 関連するPDBエントリー: 1EE5 1EJL 1IAL 1PJM 1QGK
• 分子名称: Importin alpha-2 Subunit, decamer fragment of Phospholipid scramblase 1 (3 entities in total)
• 機能のキーワード: armadillo repeat; protein:peptide complex; superhelix of helices, protein transport
• 由来する生物種: Mus musculus (house mouse); 詳細
• 細胞内の位置: Cytoplasm (By similarity): P52293; Membrane; Single-pass type II membrane protein: O15162
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47515.39

構造登録者

Chen, M.-H.,Ben-Efraim, I.,Mitrousis, G.,Walker-Kopp, N.,Sims, P.J.,Cingolani, G. (登録日: 2004-11-22, 公開日: 2005-02-01, 最終更新日: 2024-02-14)

主引用文献

Chen, M.-H.,Ben-Efraim, I.,Mitrousis, G.,Walker-Kopp, N.,Sims, P.J.,Cingolani, G.
Phospholipid Scramblase 1 Contains a Nonclassical Nuclear Localization Signal with Unique Binding Site in Importin alpha
J.Biol.Chem., 280:10599-10606, 2005

PubMed Abstract: Nuclear import of proteins containing a classical nuclear localization signal (NLS) is an energy-dependent process that requires the heterodimer importin alpha/beta. Three to six basic contiguous arginine/lysine residues characterize a classical NLS and are thought to form a basic patch on the surface of the import cargo. In this study, we have characterized the NLS of phospholipid scramblase 1 (PLSCR1), a lipid-binding protein that enters the nucleus via the nonclassical NLS (257)GKISKHWTGI(266). This import sequence lacks a contiguous stretch of positively charged residues, and it is enriched in hydrophobic residues. We have determined the 2.2 A crystal structure of a complex between the PLSCR1 NLS and the armadillo repeat core of vertebrate importin alpha. Our crystallographic analysis reveals that PLSCR1 NLS binds to armadillo repeats 1-4 of importin alpha, but its interaction partially overlaps the classical NLS binding site. Two PLSCR1 lysines occupy the canonical positions indicated as P2 and P5. Moreover, we present in vivo evidence that the critical lysine at position P2, which is essential in other known NLS sequences, is dispensable in PLSCR1 NLS. Taken together, these data provide insight into a novel nuclear localization signal that presents a distinct motif for binding to importin alpha.

PubMed: 15611084
DOI: 10.1074/jbc.M413194200

実験手法

X-RAY DIFFRACTION (2.2 Å)