1XVX

Crystal Structure of iron-loaded Yersinia enterocolitica YfuA

1XVX の概要

• エントリーDOI: 10.2210/pdb1xvx/pdb
• 分子名称: YfuA, FE (III) ION, ZINC ION, ... (6 entities in total)
• 機能のキーワード: periplasmic iron binding protein, iron binding protein
• 由来する生物種: Yersinia enterocolitica
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34484.19

構造登録者

Shouldice, S.R.,McRee, D.E.,Dougan, D.R.,Tari, L.W.,Schryvers, A.B. (登録日: 2004-10-28, 公開日: 2004-12-14, 最終更新日: 2024-02-14)

主引用文献

Shouldice, S.R.,McRee, D.E.,Dougan, D.R.,Tari, L.W.,Schryvers, A.B.
Novel Anion-independent Iron Coordination by Members of a Third Class of Bacterial Periplasmic Ferric Ion-binding Proteins
J.Biol.Chem., 280:5820-5827, 2005

PubMed Abstract: The uptake of the element iron is vital for the survival of most organisms. Numerous pathogenic Gram-negative bacteria utilize a periplasm-to-cytosol ATP-binding cassette transport pathway to transport this essential atom in to the cell. In this study, we investigated the Yersinia enterocolitica (YfuA) and Serratia marcescens (SfuA) iron-binding periplasmic proteins. We have determined the 1.8-angstroms structures of iron-loaded (YfuA) and iron-free (SfuA) forms of this class of proteins. Although the sequence of these proteins varies considerably from the other members of the transferrin structural superfamily, they adopt the same three-dimensional fold. The iron-loaded YfuA structure illustrates the unique nature of this new class of proteins in that they are able to octahedrally coordinate the ferric ion in the absence of a bound anion. The iron-free SfuA structure contains a bound citrate anion in the iron-binding cleft that tethers the N- and C-terminal domains of the apo protein and stabilizes the partially open structure.

PubMed: 15576371
DOI: 10.1074/jbc.M411238200

実験手法

X-RAY DIFFRACTION (1.53 Å)