1XU9

Crystal Structure of the Interface Closed Conformation of 11b-hydroxysteroid dehydrogenase isozyme 1

Summary for 1XU9

• Entry DOI: 10.2210/pdb1xu9/pdb
• Related: 1XU7
• Descriptor: Corticosteroid 11-beta-dehydrogenase, isozyme 1, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE, ... (5 entities in total)
• Functional Keywords: 11beta, hydroxysteroid, dehydrogenase, sdr, oxidoreductase
• Biological source: Homo sapiens (human)
• Cellular location: Endoplasmic reticulum membrane; Single-pass type II membrane protein: P28845
• Total number of polymer chains: 4
• Total formula weight: 132983.93

Authors

Hosfield, D.J.,Wu, Y.,Skene, R.J.,Hilger, M.,Jennings, A.,Snell, G.P.,Aertgeerts, K. (deposition date: 2004-10-25, release date: 2004-11-02, Last modification date: 2024-02-14)

Primary citation

Hosfield, D.J.,Wu, Y.,Skene, R.J.,Hilger, M.,Jennings, A.,Snell, G.P.,Aertgeerts, K.
Conformational Flexibility in Crystal Structures of Human 11beta-hydroxysteroid dehydrogenase type I provide insights into glucocorticoid interconversion and enzyme regulation.
J.Biol.Chem., 280:4639-4648, 2005

PubMed Abstract: Human 11beta-hydroxysteroid dehydrogenase type I (11beta-HSD1) is an ER-localized membrane protein that catalyzes the interconversion of cortisone and cortisol. In adipose tissue, excessive cortisol production through 11beta-HSD1 activity has been implicated in the pathogenesis of type II diabetes and obesity. We report here biophysical, kinetic, mutagenesis, and structural data on two ternary complexes of 11beta-HSD1. The combined results reveal flexible active site interactions relevant to glucocorticoid recognition and demonstrate how four 11beta-HSD1 C termini converge to form an as yet uncharacterized tetramerization motif. A C-terminal Pro-Cys motif is localized at the center of the tetramer and forms reversible enzyme disulfides that alter enzyme activity. Conformational flexibility at the tetramerization interface is coupled to structural changes at the enzyme active site suggesting how the central Pro-Cys motif may regulate enzyme activity. Together, the crystallographic and biophysical data provide a structural framework for understanding 11beta-HSD1 activities and will ultimately facilitate the development of specific inhibitors.

PubMed: 15513927
DOI: 10.1074/jbc.M411104200

Experimental method

X-RAY DIFFRACTION (1.55 Å)