1XU0
Solution structure of Xenopus leavis prion protein
Summary for 1XU0
| Entry DOI | 10.2210/pdb1xu0/pdb |
| Related | 1U3M 1U5L |
| NMR Information | BMRB: 6382 |
| Descriptor | prion protein (1 entity in total) |
| Functional Keywords | prion, amphibian, polymorphism, glycoprotein, membrane protein |
| Biological source | Xenopus laevis (African clawed frog) |
| Total number of polymer chains | 1 |
| Total formula weight | 15022.91 |
| Authors | Perez, D.R.,Wuthrich, K. (deposition date: 2004-10-25, release date: 2005-01-04, Last modification date: 2024-05-29) |
| Primary citation | Calzolai, L.,Lysek, D.A.,Perez, D.R.,Guntert, P.,Wuthrich, K. Prion protein NMR structures of chicken, turtle, and frog PROC.NATL.ACAD.SCI.USA, 102:651-655, 2005 Cited by PubMed Abstract: The NMR structures of the recombinant prion proteins from chicken (Gallus gallus; chPrP), the red-eared slider turtle (Trachemys scripta; tPrP), and the African clawed frog (Xenopus laevis; xlPrP) are presented. The amino acid sequences of these prion proteins show approximately 30% identity with mammalian prion proteins. All three species form the same molecular architecture as mammalian PrPC, with a long, flexibly disordered tail attached to the N-terminal end of a globular domain. The globular domain in chPrP and tPrP contains three alpha-helices, one short 3(10)-helix, and a short antiparallel beta-sheet. In xlPrP, the globular domain includes three alpha-helices and a somewhat longer beta-sheet than in the other species. The spatial arrangement of these regular secondary structures coincides closely with that of the globular domain in mammalian prion proteins. Based on the low sequence identity to mammalian PrPs, comparison of chPrP, tPrP, and xlPrP with mammalian PrPC structures is used to identify a set of essential amino acid positions for the preservation of the same PrPC fold in birds, reptiles, amphibians, and mammals. There are additional conserved residues without apparent structural roles, which are of interest for the ongoing search for physiological functions of PrPC in healthy organisms. PubMed: 15647366DOI: 10.1073/pnas.0408939102 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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