1XQI

Crystal Structure Analysis of an NDP kinase from Pyrobaculum aerophilum

1XQI の概要

• エントリーDOI: 10.2210/pdb1xqi/pdb
• 関連するPDBエントリー: 1NPK
• 分子名称: Nucleoside diphosphate kinase, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, TRIETHYLENE GLYCOL, ... (4 entities in total)
• 機能のキーワード: alpha/beta sandwich, ferredoxin fold, transferase
• 由来する生物種: Pyrobaculum aerophilum
• 細胞内の位置: Cytoplasm (By similarity): Q8ZWY4
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 66906.03

構造登録者

Pedelacq, J.D.,Waldo, G.S.,Cabantous, S.,Liong, E.C.,Berendzen, J.,Terwilliger, T.C. (登録日: 2004-10-12, 公開日: 2005-09-20, 最終更新日: 2024-10-30)

主引用文献

Pedelacq, J.D.,Waldo, G.S.,Cabantous, S.,Liong, E.C.,Terwilliger, T.C.
Structural and functional features of an NDP kinase from the hyperthermophile crenarchaeon Pyrobaculum aerophilum
Protein Sci., 14:2562-2573, 2005

PubMed Abstract: Nucleoside diphosphate (NDP) kinases are ubiquitous enzymes that transfer gamma-phosphates from nucleoside triphosphates to nucleoside diphosphates via a ping-pong mechanism. The important role of this large family of enzymes in controlling cellular functions and developmental processes along with their crystallizability has made them good candidates for structural studies. We recently determined the structure of an evolved version of an NDP kinase from Pyrobaculum aerophilum, an extreme thermophile. This NDP kinase has similarity to the 42 other NDP kinases deposited in the Protein Data Bank (PDB) but differs significantly in sequence, structure, and biophysical properties. The P. aerophilum NDP kinase sequence contains two unique segments not present in other NDP kinases, comprising residues 66-100 and 156-165. We show that deletion mutants of the P. aerophilum NDP kinase lacking either or both of these inserts have an altered substrate specificity, allowing dGTP as the phosphate donor. A structural analysis of the evolved NDP kinase in conjunction with mutagenesis experiments suggests that the substrate specificity of the P. aerophilum NDP kinase is related to the presence of these two inserts.

PubMed: 16195547
DOI: 10.1110/ps.051664205

実験手法

X-RAY DIFFRACTION (2.5 Å)