1XPH

Structure of DC-SIGNR and a portion of repeat domain 8

1XPH の概要

• エントリーDOI: 10.2210/pdb1xph/pdb
• 分子名称: CD209 antigen-like protein 1, CALCIUM ION (3 entities in total)
• 機能のキーワード: c-type lectin, carbohydrate recognition domain, repeat domain, immune system, sugar binding protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Isoform 1: Cell membrane; Single-pass type II membrane protein (Potential). Isoform 2: Cell membrane; Single-pass type II membrane protein (Potential). Isoform 3: Cell membrane; Single-pass type II membrane protein (Potential). Isoform 5: Secreted (Potential). Isoform 6: Secreted (Potential). Isoform 7: Secreted (Potential). Isoform 10: Secreted (Potential): Q9H2X3
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17603.30

構造登録者

Snyder, G.A.,Colonna, M.,Sun, P.D. (登録日: 2004-10-08, 公開日: 2005-04-19, 最終更新日: 2024-11-13)

主引用文献

Snyder, G.A.,Colonna, M.,Sun, P.D.
The structure of DC-SIGNR with a portion of its repeat domain lends insights to modeling of the receptor tetramer.
J.Mol.Biol., 347:979-989, 2005

PubMed Abstract: The dendritic cell-specific ICAM-3 non-integrin (DC-SIGN) and its close relative DC-SIGNR recognize various glycoproteins, both pathogenic and cellular, through the receptor lectin domain-mediated carbohydrate recognition. While the carbohydrate-recognition domains (CRD) exist as monomers and bind individual carbohydrates with low affinity and are permissive in nature, the full-length receptors form tetramers through their repeat domain and recognize specific ligands with high affinity. To understand the tetramer-based ligand binding avidity, we determined the crystal structure of DC-SIGNR with its last repeat region. Compared to the carbohydrate-bound CRD structure, the structure revealed conformational changes in the calcium and carbohydrate coordination loops of CRD, an additional disulfide bond between the N and the C termini of the CRD, and a helical conformation for the last repeat. On the basis of the current crystal structure and other published structures with sequence homology to the repeat domain, we generated a tetramer model for DC-SIGN/R using homology modeling and propose a ligand-recognition index to identify potential receptor ligands.

PubMed: 15784257
DOI: 10.1016/j.jmb.2005.01.063

実験手法

X-RAY DIFFRACTION (1.41 Å)