1XOX

SOLUTION STRUCTURE OF HUMAN SURVIVIN

Summary for 1XOX

• Entry DOI: 10.2210/pdb1xox/pdb
• NMR Information: BMRB: 6342
• Descriptor: Apoptosis inhibitor survivin, ZINC ION (2 entities in total)
• Functional Keywords: bir domain; apoptosis, apoptosis
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm: O15392
• Total number of polymer chains: 2
• Total formula weight: 27085.48

Authors

Sun, C.,Nettesheim, D.,Liu, Z.,Olejniczak, E.T. (deposition date: 2004-10-07, release date: 2005-01-18, Last modification date: 2024-05-22)

Primary citation

Sun, C.,Nettesheim, D.,Liu, Z.,Olejniczak, E.T.
Solution structure of human survivin and its binding interface with smac/diablo
Biochemistry, 44:11-17, 2005

PubMed Abstract: NMR studies of the antiapoptotic protein survivin have been used to determine the homodimer interface of the protein in solution and to identify residues of the protein that interact with Smac/Diablo. In solution, survivin(1-120) forms a bow-tie-shaped dimer whose interface is composed of its N-terminal residues as well as residues connecting its BIR domain to the C-terminal alpha helix. The solution structure resolves the controversy regarding the two possible dimer interfaces for survivin observed in X-ray crystal structures. The structural basis for the interaction between survivin and Smac/Diablo was also investigated. When Smac/Diablo or N-terminal Smac/Diablo peptide analogues are added to a solution of survivin, specific residues near alpha4 and beta3 are perturbed. NMR experiments indicate that the peptides bind across the third beta-strand of survivin in a manner similar to the way Smac/Diablo peptides bind to the BIR3 domain of X-linked IAP (XIAP).

PubMed: 15628841
DOI: 10.1021/bi0485171

Experimental method

SOLUTION NMR