1XOD

Crystal structure of X. tropicalis Spred1 EVH-1 domain

1XOD の概要

• エントリーDOI: 10.2210/pdb1xod/pdb
• 分子名称: Spred1, GLYCEROL (3 entities in total)
• 機能のキーワード: spred, sprouty, evh1, peptide-binding, signaling protein
• 由来する生物種: Xenopus tropicalis (western clawed frog)
• 細胞内の位置: Membrane; Peripheral membrane protein (By similarity): Q66JG9
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 26033.17

構造登録者

Harmer, N.J.,Sivak, J.M.,Amaya, E.,Blundell, T.L. (登録日: 2004-10-06, 公開日: 2005-01-25, 最終更新日: 2023-08-23)

主引用文献

Harmer, N.J.,Sivak, J.M.,Amaya, E.,Blundell, T.L.
1.15A Crystal structure of the X. tropicalis Spred1 EVH1 domain suggests a fourth distinct peptide-binding mechanism within the EVH1 family
Febs Lett., 579:1161-1166, 2005

PubMed Abstract: The recently described Spred protein family has been implicated in the modulation of receptor tyrosine kinase signalling. We report the crystal structure of the Enabled/vasodilator-stimulated phosphoprotein homology-1 (EVH1) domain from Xenopus tropicalis Spred1, solved to 1.15 A resolution. This structure confirms that the Spred EVH1 adopts the pleckstrin-homology fold, with a similar secondary structure to Enabled. A translation of one of the peptide-binding groove beta-strands narrows this groove, whilst one end of the groove shows structural flexibility. We propose that Spred1 will bind peptides that are less proline-rich than other EVH1 domains, with conformational changes indicating an induced fit.

PubMed: 15710406
DOI: 10.1016/j.febslet.2004.11.114

実験手法

X-RAY DIFFRACTION (1.15 Å)