1XHP
Solution Structure of the Extended U6 ISL as Observed in the U2/U6 complex from Saccharomyces cerevisiae
Summary for 1XHP
| Entry DOI | 10.2210/pdb1xhp/pdb |
| Related | 1KXK 1R2P 1SY4 |
| NMR Information | BMRB: 6320 |
| Descriptor | U6 snRNA (1 entity in total) |
| Functional Keywords | u6 rna, rna stem-loop, penta-loop, internal bulge, metal binding site, rna |
| Total number of polymer chains | 1 |
| Total formula weight | 10240.13 |
| Authors | Sashital, D.G.,Cornilescu, G.,Butcher, S.E. (deposition date: 2004-09-20, release date: 2004-11-23, Last modification date: 2024-05-29) |
| Primary citation | Sashital, D.G.,Cornilescu, G.,McManus, C.J.,Brow, D.A.,Butcher, S.E. U2-U6 RNA folding reveals a group II intron-like domain and a four-helix junction Nat.Struct.Mol.Biol., 11:1237-1242, 2004 Cited by PubMed Abstract: Intron removal in nuclear precursor mRNA is catalyzed through two transesterification reactions by a multi-megaDalton ribonucleoprotein machine called the spliceosome. A complex between U2 and U6 small nuclear RNAs is a core component of the spliceosome. Here we present an NMR structural analysis of a protein-free U2-U6 complex from Saccharomyces cerevisiae. The observed folding of the U2-U6 complex is a four-helix junction, in which the catalytically important AGC triad base-pairs only within U6 and not with U2. The base-pairing of the AGC triad extends the U6 intramolecular stem-loop (U6 ISL), and the NMR structure of this extended U6 ISL reveals structural similarities with domain 5 of group II self-splicing introns. The observed conformation of the four-helix junction could be relevant to the first, but not the second, step of splicing and may help to position the U6 ISL adjacent to the 5' splice site. PubMed: 15543154DOI: 10.1038/nsmb863 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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