1XHP

Solution Structure of the Extended U6 ISL as Observed in the U2/U6 complex from Saccharomyces cerevisiae

1XHP の概要

• エントリーDOI: 10.2210/pdb1xhp/pdb
• 関連するPDBエントリー: 1KXK 1R2P 1SY4
• NMR情報: BMRB: 6320
• 分子名称: U6 snRNA (1 entity in total)
• 機能のキーワード: u6 rna, rna stem-loop, penta-loop, internal bulge, metal binding site, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10240.13

構造登録者

Sashital, D.G.,Cornilescu, G.,Butcher, S.E. (登録日: 2004-09-20, 公開日: 2004-11-23, 最終更新日: 2024-05-29)

主引用文献

Sashital, D.G.,Cornilescu, G.,McManus, C.J.,Brow, D.A.,Butcher, S.E.
U2-U6 RNA folding reveals a group II intron-like domain and a four-helix junction
Nat.Struct.Mol.Biol., 11:1237-1242, 2004

PubMed Abstract: Intron removal in nuclear precursor mRNA is catalyzed through two transesterification reactions by a multi-megaDalton ribonucleoprotein machine called the spliceosome. A complex between U2 and U6 small nuclear RNAs is a core component of the spliceosome. Here we present an NMR structural analysis of a protein-free U2-U6 complex from Saccharomyces cerevisiae. The observed folding of the U2-U6 complex is a four-helix junction, in which the catalytically important AGC triad base-pairs only within U6 and not with U2. The base-pairing of the AGC triad extends the U6 intramolecular stem-loop (U6 ISL), and the NMR structure of this extended U6 ISL reveals structural similarities with domain 5 of group II self-splicing introns. The observed conformation of the four-helix junction could be relevant to the first, but not the second, step of splicing and may help to position the U6 ISL adjacent to the 5' splice site.

PubMed: 15543154
DOI: 10.1038/nsmb863

実験手法

SOLUTION NMR