1XFW

Crystal structure of anthrax edema factor (EF) in complex with calmodulin and 3'5' cyclic AMP (cAMP)

1XFW の概要

• エントリーDOI: 10.2210/pdb1xfw/pdb
• 関連するPDBエントリー: 1XFU 1XFV 1XFX 1XFY 1XFZ
• 分子名称: Calmodulin-sensitive adenylate cyclase, Calmodulin 2, MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: protein-protein interaction, lyase-metal binding protein complex, lyase/metal binding protein
• 由来する生物種: Bacillus anthracis; 詳細
• 細胞内の位置: Secreted: P40136
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 644725.22

構造登録者

Shen, Y.,Zhukovskaya, N.L.,Guo, Q.,Florian, J.,Tang, W.J. (登録日: 2004-09-15, 公開日: 2005-05-03, 最終更新日: 2024-02-14)

主引用文献

Shen, Y.,Zhukovskaya, N.L.,Guo, Q.,Florian, J.,Tang, W.J.
Calcium-independent calmodulin binding and two-metal-ion catalytic mechanism of anthrax edema factor.
EMBO J., 24:929-941, 2005

PubMed Abstract: Edema factor (EF), a key anthrax exotoxin, has an anthrax protective antigen-binding domain (PABD) and a calmodulin (CaM)-activated adenylyl cyclase domain. Here, we report the crystal structures of CaM-bound EF, revealing the architecture of EF PABD. CaM has N- and C-terminal domains and each domain can bind two calcium ions. Calcium binding induces the conformational change of CaM from closed to open. Structures of the EF-CaM complex show how EF locks the N-terminal domain of CaM into a closed conformation regardless of its calcium-loading state. This represents a mechanism of how CaM effector alters the calcium affinity of CaM and uncouples the conformational change of CaM from calcium loading. Furthermore, structures of EF-CaM complexed with nucleotides show that EF uses two-metal-ion catalysis, a prevalent mechanism in DNA and RNA polymerases. A histidine (H351) further facilitates the catalysis of EF by activating a water to deprotonate 3'OH of ATP. Mammalian adenylyl cyclases share no structural similarity with EF and they also use two-metal-ion catalysis, suggesting the catalytic mechanism-driven convergent evolution of two structurally diverse adenylyl cyclases.

PubMed: 15719022
DOI: 10.1038/sj.emboj.7600574

実験手法

X-RAY DIFFRACTION (3.4 Å)