1XFT

Synchrotron X-ray Powder Diffraction Study of Hexagonal Turkey Egg-white Lysozyme

1XFT の概要

• エントリーDOI: 10.2210/pdb1xft/pdb
• 関連するPDBエントリー: 1JA6 1TEW 2LZ2
• 分子名称: Lysozyme C (1 entity in total)
• 機能のキーワード: powder diffraction, lysozyme, x-rays, hydrolase
• 由来する生物種: Meleagris gallopavo (turkey)
• 細胞内の位置: Secreted: P00703
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14228.10

構造登録者

Margiolaki, I.,Wright, J.P. (登録日: 2004-09-15, 公開日: 2005-04-05, 最終更新日: 2024-11-20)

主引用文献

Margiolaki, I.,Wright, J.P.,Fitch, A.N.,Fox, G.C.,Von Dreele, R.B.
Synchrotron X-ray powder diffraction study of hexagonal turkey egg-white lysozyme.
Acta Crystallogr.,Sect.D, 61:423-432, 2005

PubMed Abstract: The structure of turkey egg-white lysozyme (TEWL) has been refined from high-resolution X-ray powder diffraction data. The sample was rapidly obtained as a polycrystalline precipitate at high protein concentration using 0.5 M NaCl solvent pH 6 and was deposited in the PDB with code 1xft. The diffraction data were collected at room temperature. Molecular replacement was shown to give a suitable starting point for refinement, illustrating that powder data can be sufficient for this approach. Crystallographic models were then refined by combined Rietveld and stereochemical restraint analysis of the powder data (d(min) = 3.35 A), resulting in the extraction of reliable lattice parameters and the refinement of the molecular conformation at room temperature. The structure is hexagonal [space group P6(1)22, unit-cell parameters a = 71.0862 (3), c = 85.0276 (5) A] with 12 symmetry-related molecules in the unit cell, in agreement with previous studies. The results of our analysis are indicative of specific amino acids being disordered at this temperature. Upon cooling, a sudden drop in the lattice parameters at approximately 250 K is observed concurrently with the freezing of the mother liquor. The observation of severe peak broadening below this temperature indicates strain effects accompanying the freezing transition, which are found to be reversible. Finally, a correlation between the unit-cell parameters and the pH of the buffer solution is evident, in a similar manner to earlier observations on HEWL.

PubMed: 15805597
DOI: 10.1107/S0907444905001393

実験手法

POWDER DIFFRACTION