1XF1

Structure of C5a peptidase- a key virulence factor from Streptococcus

1XF1 の概要

• エントリーDOI: 10.2210/pdb1xf1/pdb
• 分子名称: C5a peptidase, ACETATE ION, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: c5a peptidase, hydrolase
• 由来する生物種: Streptococcus pyogenes
• 細胞内の位置: Secreted, cell wall; Peptidoglycan-anchor (Potential): P15926
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 207343.13

構造登録者

Brown, C.K.,Gu, Z.Y.,Cleary, P.P.,Matsuka, Y.,Olmstead, S.,Ohlendorf, D.H.,Earhart, C.A. (登録日: 2004-09-13, 公開日: 2005-11-22, 最終更新日: 2024-10-30)

主引用文献

Brown, C.K.,Gu, Z.Y.,Matsuka, Y.V.,Purushothaman, S.S.,Winter, L.A.,Cleary, P.P.,Olmsted, S.B.,Ohlendorf, D.H.,Earhart, C.A.
Structure of the streptococcal cell wall C5a peptidase
Proc.Natl.Acad.Sci.Usa, 102:18391-18396, 2005

PubMed Abstract: The structure of a cell surface enzyme from a gram-positive pathogen has been determined to 2-A resolution. Gram-positive pathogens have a thick cell wall to which proteins and carbohydrate are covalently attached. Streptococcal C5a peptidase (SCP), is a highly specific protease and adhesin/invasin. Structural analysis of a 949-residue fragment of the [D130A,S512A] mutant of SCP from group B Streptococcus (S. agalactiae, SCPB) revealed SCPB is composed of five distinct domains. The N-terminal subtilisin-like protease domain has a 134-residue protease-associated domain inserted into a loop between two beta-strands. This domain also contains one of two Arg-Gly-Asp (RGD) sequences found in SCPB. At the C terminus are three fibronectin type III (Fn) domains. The second RGD sequence is located between Fn1 and Fn2. Our analysis suggests that SCP binding to integrins by the RGD motifs may stabilize conformational changes required for substrate binding.

PubMed: 16344483
DOI: 10.1073/pnas.0504954102

実験手法

X-RAY DIFFRACTION (1.9 Å)