1XE3

Crystal Structure of purine nucleoside phosphorylase DeoD from Bacillus anthracis

Summary for 1XE3

• Entry DOI: 10.2210/pdb1xe3/pdb
• Descriptor: purine nucleoside phosphorylase, CHLORIDE ION (3 entities in total)
• Functional Keywords: purine nucleoside phosphorylase, deod, spine, structural genomics, structural proteomics in europe, transferase
• Biological source: Bacillus anthracis
• Total number of polymer chains: 6
• Total formula weight: 161562.71

Authors

Grenha, R.,Levdikov, V.M.,Fogg, M.,Blagova, E.V.,Brannigan, J.A.,Wilkinson, A.J.,Wilson, K.S.,Structural Proteomics in Europe (SPINE) (deposition date: 2004-09-09, release date: 2004-10-26, Last modification date: 2023-08-23)

Primary citation

Grenha, R.,Levdikov, V.M.,Fogg, M.J.,Blagova, E.V.,Brannigan, J.A.,Wilkinson, A.J.,Wilson, K.S.
Structure of purine nucleoside phosphorylase (DeoD) from Bacillus anthracis.
Acta Crystallogr.,Sect.F, 61:459-462, 2005

PubMed Abstract: Protein structures from the causative agent of anthrax (Bacillus anthracis) are being determined as part of a structural genomics programme. Amongst initial candidates for crystallographic analysis are enzymes involved in nucleotide biosynthesis, since these are recognized as potential targets in antibacterial therapy. Purine nucleoside phosphorylase is a key enzyme in the purine-salvage pathway. The crystal structure of purine nucleoside phosphorylase (DeoD) from B. anthracis has been solved by molecular replacement at 2.24 A resolution and refined to an R factor of 18.4%. This is the first report of a DeoD structure from a Gram-positive bacterium.

PubMed: 16511068
DOI: 10.1107/S174430910501095X

Experimental method

X-RAY DIFFRACTION (2.24 Å)