1XDT

COMPLEX OF DIPHTHERIA TOXIN AND HEPARIN-BINDING EPIDERMAL GROWTH FACTOR

Summary for 1XDT

• Entry DOI: 10.2210/pdb1xdt/pdb
• Descriptor: DIPHTHERIA TOXIN, HEPARIN-BINDING EPIDERMAL GROWTH FACTOR (3 entities in total)
• Functional Keywords: complex (toxin-growth factor), diphtheria toxin, receptor, heparin-binding epidermal growth factor, epidermal growth factor, complex (toxin-growth factor) complex, complex (toxin/growth factor)
• Biological source: Corynebacterium diphtheriae; More
• Cellular location: Heparin-binding EGF-like growth factor: Secreted, extracellular space. Proheparin-binding EGF-like growth factor: Cell membrane; Single-pass type I membrane protein: Q99075
• Total number of polymer chains: 2
• Total formula weight: 67338.87

Authors

Louie, G.V.,Yang, W.,Bowman, M.E.,Choe, S. (deposition date: 1997-11-18, release date: 1998-02-25, Last modification date: 2024-10-23)

Primary citation

Louie, G.V.,Yang, W.,Bowman, M.E.,Choe, S.
Crystal structure of the complex of diphtheria toxin with an extracellular fragment of its receptor.
Mol.Cell, 1:67-78, 1997

PubMed Abstract: We describe the crystal structure at 2.65 A resolution of diphtheria toxin (DT) complexed 1:1 with a fragment of its cell-surface receptor, the precursor of heparin-binding epidermal-growth-factor-like growth factor (HBEGF). HBEGF in the complex has the typical EGF-like fold and packs its principal beta hairpin against the face of a beta sheet in the receptor-binding domain of DT. The interface has a predominantly hydrophobic core, and polar interactions are formed at the periphery. The structure of the complex suggests that part of the membrane anchor of the receptor can interact with a hinge region of DT. The toxin molecule is thereby induced to form an open conformation conducive to membrane insertion. The structure provides a basis for altering the binding specificity of the toxin, and may also serve as a model for other EGF-receptor interactions.

PubMed: 9659904
DOI: 10.1016/S1097-2765(00)80008-8

Experimental method

X-RAY DIFFRACTION (2.65 Å)