1XAE

Crystal structure of wild type yellow fluorescent protein zFP538 from Zoanthus

1XAE の概要

• エントリーDOI: 10.2210/pdb1xae/pdb
• 関連するPDBエントリー: 1XA9
• 分子名称: fluorescent protein FP538, BETA-MERCAPTOETHANOL (3 entities in total)
• 機能のキーワード: fluorescent protein, beta-can, beta-barrel, luminescent protein
• 由来する生物種: Zoanthus sp.
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52552.66

構造登録者

Remington, S.J.,Wachter, R.M.,Yarbrough, D.K.,Branchaud, B.,Anderson, D.C.,Kallio, K.,Lukyanov, K.A. (登録日: 2004-08-25, 公開日: 2005-02-08, 最終更新日: 2026-03-18)

主引用文献

Remington, S.J.,Wachter, R.M.,Yarbrough, D.K.,Branchaud, B.,Anderson, D.C.,Kallio, K.,Lukyanov, K.A.
zFP538, a yellow-fluorescent protein from Zoanthus, contains a novel three-ring chromophore.
Biochemistry, 44:202-212, 2005

PubMed Abstract: Crystal structures of the tetrameric yellow-fluorescent protein zFP538 from the button polyp Zoanthus sp. and a green-emitting mutant (K66M) are presented. The atomic models have been refined at 2.7 and 2.5 A resolution, with final crystallographic R factors of 0.206 (R(free) = 0.255) and 0.190 (R(free) = 0.295), respectively, and have excellent stereochemistry. The fold of the protomer is very similar to that of green (GFP) and red (DsRed) fluorescent proteins; however, evidence from crystallography and mass spectrometry suggests that zFP538 contains a three-ring chromophore derived from that of GFP. The yellow-emitting species (lambda(em)(max) = 538 nm) is proposed to result from a transimination reaction in which a transiently appearing DsRed-like acylimine is attacked by the terminal amino group of lysine 66 to form a new six-membered ring, cleaving the polypeptide backbone at the 65-66 position. This extends the chromophore conjugation by an additional double bond compared to GFP, lowering the absorption and emission frequencies. Substitution of lysine 66 with aspartate or glutamate partially converts zFP538 into a red-fluorescent protein, providing additional support for an acylimine intermediate. The diverse and unexpected roles of the side chain at position 66 give new insight into the chemistry of chromophore maturation in the extended family of GFP-like proteins.

PubMed: 15628861
DOI: 10.1021/bi048383r

実験手法

X-RAY DIFFRACTION (2.7 Å)