1X9T

The crystal structure of human adenovirus 2 penton base in complex with an ad2 N-terminal fibre peptide

1X9T の概要

• エントリーDOI: 10.2210/pdb1x9t/pdb
• 関連するPDBエントリー: 1X9P
• 分子名称: Penton protein, N-terminal peptide of Fiber protein, N-DODECYL-N,N-DIMETHYL-3-AMMONIO-1-PROPANESULFONATE (3 entities in total)
• 機能のキーワード: jellyroll domain, insertion domain, anti-parallel beta sheets, virus like particle-peptide complex, virus like particle/peptide
• 由来する生物種: Human adenovirus 2; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 61414.58

構造登録者

Zubieta, C.,Schoehn, G.,Chroboczek, J.,Cusack, S. (登録日: 2004-08-24, 公開日: 2005-01-18, 最終更新日: 2024-04-03)

主引用文献

Zubieta, C.,Schoehn, G.,Chroboczek, J.,Cusack, S.
The structure of the human adenovirus 2 penton
Mol.Cell, 17:121-135, 2005

PubMed Abstract: The adenovirus penton, a noncovalent complex of the pentameric penton base and trimeric fiber proteins, comprises the vertices of the adenovirus capsid and contains all necessary components for viral attachment and internalization. The 3.3 A resolution crystal structure of human adenovirus 2 (hAd2) penton base shows that the monomer has a basal jellyroll domain and a distal irregular domain formed by two long insertions, a similar topology to the adenovirus hexon. The Arg-Gly-Asp (RGD) motif, required for interactions with cellular integrins, occurs on a flexible surface loop. The complex of penton base with bound N-terminal fiber peptide, determined at 3.5 A resolution, shows that the universal fiber motif FNPVYPY binds at the interface of adjacent penton base monomers and results in a localized structural rearrangement in the insertion domain of the penton base. These results give insight into the structure and assembly of the adenovirus capsid and will be of use for gene-therapy applications.

PubMed: 15629723
DOI: 10.1016/j.molcel.2004.11.041

実験手法

X-RAY DIFFRACTION (3.5 Å)