1WPI

Solution NMR Structure of Protein YKR049C from Saccharomyces cerevisiae. Ontario Centre for Structural Proteomics target YST0250_1_133; Northeast Structural Genomics Consortium YTYst250

1WPI の概要

• エントリーDOI: 10.2210/pdb1wpi/pdb
• 分子名称: Hypothetical 15.6 kDa protein in NAP1-TRK2 intergenic region (1 entity in total)
• 機能のキーワード: structural genomics, ocsp, nesg, protein structure initiative, psi-2, northeast structural genomics consortium, unknown function
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Mitochondrion: P36141
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15672.04

構造登録者

Jung, J.W.,Yee, A.,Arrowsmith, C.H.,Lee, W.,Northeast Structural Genomics Consortium (NESG) (登録日: 2004-09-03, 公開日: 2005-09-13, 最終更新日: 2024-05-29)

主引用文献

Jung, J.W.,Yee, A.,Wu, B.,Arrowsmith, C.H.,Lee, W.
Solution structure of YKR049C, a putative redox protein from Saccharomyces cerevisiae
J.Biochem.Mol.Biol., 38:550-554, 2005

PubMed Abstract: YKR049C is a mitochondrial protein in Saccharomyces cerevisiae that is conserved among yeast species, including Candida albicans. However, no biological function for YKR049C has been ascribed based on its primary sequence information. In the present study, NMR spectroscopy was used to determine the putative biological function of YKR049C based on its solution structure. YKR049C shows a well-defined thioredoxin fold with a unique insertion of helices between two beta-strands. The central beta-sheet divides the protein into two parts; a unique face and a conserved face. The 'unique face' is located between beta2 and beta3. Interestingly, the sequences most conserved among YKR049C families are found on this 'unique face', which incorporates L109 to E114. The side chains of these conserved residues interact with residues on the helical region with a stretch of hydrophobic surface. A putative active site composed by two short helices and a single Cys97 was also well observed. Our findings suggest that YKR049C is a redox protein with a thioredoxin fold containing a single active cysteine.

PubMed: 16202234

実験手法

SOLUTION NMR