1WPD

Evidence for domain-specific recognition of SK and Kv channels by MTX and HsTx1 scorpion toxins

1WPD の概要

• エントリーDOI: 10.2210/pdb1wpd/pdb
• NMR情報: BMRB: 6347
• 分子名称: Potassium channel toxin alpha-KTx 6.2,Potassium channel toxin alpha-KTx 6.3 (1 entity in total)
• 機能のキーワード: neurotoxin, chimera, toxin
• 由来する生物種: Scorpio palmatus (Israeli golden scorpion); 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3801.56

構造登録者

Regaya, I.,Beeton, C.,Ferrat, G.,Andreotti, N.,Chandy, G.K.,Darbon, H.,De Waard, M.,Sabatier, J.M. (登録日: 2004-09-01, 公開日: 2004-10-19, 最終更新日: 2024-10-16)

主引用文献

Regaya, I.,Beeton, C.,Ferrat, G.,Andreotti, N.,Darbon, H.,De Waard, M.,Sabatier, J.M.
Evidence for domain-specific recognition of SK and Kv channels by MTX and HsTx1 scorpion toxins
J.Biol.Chem., 279:55690-55696, 2004

PubMed Abstract: Maurotoxin (MTX) and HsTx1 are two scorpion toxins belonging to the alpha-KTx6 structural family. These 34-residue toxins, cross-linked by four disulfide bridges, share 59% sequence identity and fold along the classical alpha/beta scaffold. Despite these structural similarities, they fully differ in their pharmacological profiles. MTX is highly active on small (SK) and intermediate (IK) conductance Ca(2+)-activated (K(+)) channels and on voltage-gated Kv1.2 channel, whereas HsTx1 potently blocks voltage-gated Kv1.1 and Kv1.3 channels only. Here, we designed and chemically produced MTX-HsTx1, a chimera of both toxins that contains the N-terminal helical region of MTX (sequence 1-16) and the C-terminal beta-sheet region of HsTx1 (sequence 17-34). The three-dimensional structure of the peptide in solution was solved by (1)H NMR. MTX-HsTx1 displays the activity of MTX on SK channel, whereas it exhibits the pharmacological profile of HsTx1 on Kv1.1, Kv1.2, Kv1.3, and IK channels. These data demonstrate that the helical region of MTX exerts a key role in SK channel recognition, whereas the beta-sheet region of HsTx1 is crucial for activity on all other channel types tested.

PubMed: 15498765
DOI: 10.1074/jbc.M410055200

実験手法

SOLUTION NMR